Human Colon Cancer Primer Library I

Contains 88 primer sets directed against colon cancer carcinogenesis related genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul; - 10 uM;)

Perform up to 100 PCR arrays (based on 20 ul; assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ACVR2A activin A receptor, type IIA

ADAM17 ADAM metallopeptidase domain 17

AKT1 v-akt murine thymoma viral oncogene homolog 1

ALX4 ALX homeobox 4

APC adenomatous polyposis coli

AXIN1 axin 1

AXIN2 axin 2

BGN biglycan

BMP1 bone morphogenetic protein 1

BMP2 bone morphogenetic protein 2

BMP3 bone morphogenetic protein 3

BMP4 bone morphogenetic protein 4

BMPR1 bone morphogenetic protein receptor, type I

BMPR2 bone morphogenetic protein receptor, type II

BRAF B-Raf proto-oncogene, serine/threonine kinase

CDKN2A cyclin-dependent kinase inhibitor 2A

CDX2 caudal type homeobox 2

CEACAM1 carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)

CEACAM5 carcinoembryonic antigen-related cell adhesion molecule 5

CHRD chordin

CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa

DHH desert hedgehog

DLL1 delta-like 1 (Drosophila)

DLL4 delta-like 4 (Drosophila)

DVL1 dishevelled segment polarity protein 1

DVL2 dishevelled segment polarity protein 2

EGFR epidermal growth factor receptor

FAP fibroblast activation protein, alpha

FLT1 fms-related tyrosine kinase 1

FZD1 frizzled class receptor 1

FZD3 frizzled class receptor 3

GADD45B growth arrest and DNA-damage-inducible, beta

GLI1 GLI family zinc finger 1

GLI2 GLI family zinc finger 2

GLI3 GLI family zinc finger 3

GSK3B glycogen synthase kinase 3 beta

HHAT hedgehog acyltransferase

HLTF helicase-like transcription factor

IGFBP7 insulin-like growth factor binding protein 7

INHBA inhibin, beta A

ITGA4 integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)

JAG1 jagged 1

JAG2 jagged 2

KRAS Kirsten rat sarcoma viral oncogene homolog

MAPK1 mitogen-activated protein kinase 1

MAPK3 mitogen-activated protein kinase 3

MAPK8 mitogen-activated protein kinase 8

MGMT O-6-methylguanine-DNA methyltransferase

MKI67 marker of proliferation Ki-67

MLH1 mutL homolog 1

MSH2 mutS homolog 2

MSH6 mutS homolog 6

MTOR mechanistic target of rapamycin (serine/threonine kinase)

MYBL2 v-myb avian myeloblastosis viral oncogene homolog- like 2

MYC v-myc avian myeloblastosis viral oncogene homolog

NEUROG1 neurogenin 1

NGFR nerve growth factor receptor NOG noggin

NOTCH1 notch 1

NOTCH2 notch 2

PIK3CA phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha

PMS2 PMS2 postmeiotic segregation increased 2 (S. cerevisiae)

PRKCA protein kinase C, alpha

PRKCB protein kinase C, beta

PTCH1 patched 1

PTCH2 patched 2

PTEN phosphatase and tensin homolog

RASSF2 Ras association (RalGDS/AF-6) domain family member 2

SEPT9 septin 9

SFRP2 secreted frizzled-related protein 2

SLC5A8 solute carrier family 5 (sodium/monocarboxylate cotransporter), member 8

SMAD1 SMAD family member 1

SMAD3 SMAD family member 3

SMAD4 SMAD family member 4

SMO smoothened, frizzled class receptor

SOX9 SRY (sex determining region Y)-box 9

STAT3 signal transducer and activator of transcription 3 (acute-phase response factor)

TGFB2 transforming growth factor, beta 2

TGFBR2 transforming growth factor, beta receptor II (70/80kDa)

TP53 tumor protein p53

TYMS thymidylate synthetase

VEGFA vascular endothelial growth factor A

VIM vimentin

WIF1 WNT inhibitory factor 1

WNT1 wingless-type MMTV integration site family, member 1

WNT3 wingless-type MMTV integration site family, member 3

WNT4 wingless-type MMTV integration site family, member 4

WNT5 wingless-type MMTV integration site family, member 5

VIM vimentin

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a