PCR arrays provide a simple and powerful method to analyze gene expression for hundreds of genes simultaneously. These assays require access to a 96 or 384-well real time PCR instrument and a plate of primer sets designed to amplify specific cDNAs representing a specified pathway or group of genes/biomarkers.
The spreadsheets shown at PCRArray.com may be downloaded and used as a template for quantitating PCR array data based on normalization to housekeeping gene expression or "all gene" expression. One can replace the specific gene lists and well locations with your own data and add/remove columns based on the specific number of arrays, replicates and samples from your own experiments. These spreadsheets compare one set of samples to another for one particular experimental variable.