Real Time Primers offers PCR primer libraries focused on disease related gene expression, including cancer, neurological and developmental disorders. These libraries group genes linked to the molecular mechanisms of each disease so that researchers can profile disease pathways in a single real time PCR array experiment. Select a disease category to see the primer libraries available.
Autism spectrum disorder (ASD) is a neurodevelopmental condition characterized by differences in social communication and by restricted, repetitive behaviors. ASD has a strong genetic component, and many of the genes implicated encode proteins involved in synapse formation and function, neuronal signaling, chromatin remodeling and transcriptional regulation. Examples include synaptic scaffolding and adhesion genes such as SHANK3, NLGN3, NLGN4X and NRXN1, as well as regulators such as MECP2, FMR1, PTEN, TSC1/TSC2 and CHD8. Gene expression profiling helps researchers explore how these networks are altered in neuronal and other cell models of ASD. |
Breast cancer is the most common cancer in women worldwide and is classified into molecular subtypes based on expression of the estrogen receptor (ESR1), progesterone receptor (PGR) and HER2 (ERBB2), including luminal A, luminal B, HER2-enriched and triple negative/basal-like tumors. Key pathways in breast cancer include estrogen receptor signaling, ERBB/HER2 and EGFR signaling, PI3K-AKT-mTOR, cell cycle regulation through cyclin D1 and CDK4/6, and DNA repair genes such as BRCA1 and BRCA2. Gene expression profiling by real time PCR is widely used to study these pathways and to characterize tumor subtypes. |