Breast cancer is the most common cancer in women worldwide and is classified into molecular subtypes based on expression of the estrogen receptor (ESR1), progesterone receptor (PGR) and HER2 (ERBB2), including luminal A, luminal B, HER2-enriched and triple negative/basal-like tumors. Key pathways in breast cancer include estrogen receptor signaling, ERBB/HER2 and EGFR signaling, PI3K-AKT-mTOR, cell cycle regulation through cyclin D1 and CDK4/6, and DNA repair genes such as BRCA1 and BRCA2. Gene expression profiling by real time PCR is widely used to study these pathways and to characterize tumor subtypes.
A ready-to-use Human Breast Cancer AI PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against breast cancer genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: AKT1 v-akt murine thymoma viral oncogene homolog 1 AR androgen receptor ATM ATM serine/threonine kinase AURKA aurora kinase A AURKB aurora kinase B BRCA1 breast cancer 1, early onset BRCA2 breast cancer 2, early onset BRIP1 BRCA1 Interacting Helicase 1 BUB1 BUB1 mitotic checkpoint serine/threonine kinase BUB1B BUB1 mitotic checkpoint serine/threonine kinase B CCNB1 cyclin B1 CCNB2 cyclin B2 CCNE1 cyclin E1 CCNE2 cyclin E2 CDC20 cell division cycle 20 CDH1 cadherin 1, type 1, E-cadherin (epithelial) CDK2 cyclin-dependent kinase 2 CDK4 cyclin-dependent kinase 4 CDK6 cyclin-dependent kinase 6 CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1) CDKN2A cyclin-dependent kinase inhibitor 2A CDKN2B cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) CHEK1 checkpoint kinase 1 CHEK2 checkpoint kinase 2 COMT catechol-O-methyltransferase CYP1A1 cytochrome P450, family 1, subfamily A, polypeptide 1 CYP1B1 cytochrome P450, family 1, subfamily B, polypeptide 1 DLL1 delta-like 1 (Drosophila) DLL3 delta-like 3 (Drosophila) DLL4 delta-like 4 (Drosophila) ERBB2 erb-b2 receptor tyrosine kinase 2 (HER2) ERBB3 erb-b2 receptor tyrosine kinase 3 ERBB4 erb-b2 receptor tyrosine kinase 4 ESR1 estrogen receptor 1 ESR2 estrogen receptor 2 (ER beta) FANCA Fanconi anemia, complementation group A FANCC Fanconi anemia, complementation group C FANCD2 Fanconi anemia, complementation group D2 FANCE Fanconi anemia, complementation group E FANCF Fanconi anemia, complementation group F FANCG Fanconi anemia, complementation group G FGFR1 fibroblast growth factor receptor 1 FGFR2 fibroblast growth factor receptor 2 FGFR3 fibroblast growth factor receptor 3 FGFR4 fibroblast growth factor receptor 4 FOXA1 forkhead box A1 GATA3 GATA binding protein 3 GLI1 GLI family zinc finger 1 GLI2 GLI family zinc finger 2 GLI3 GLI family zinc finger 3 GPX1 glutathione peroxidase 1 GSTP1 glutathione S-transferase pi 1 HGF hepatocyte growth factor (hepapoietin A; scatter factor) IL10 interleukin 10 IL6 interleukin 6 JAG1 jagged 1 JAG2 jagged 2 KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog MAP2K4 mitogen-activated protein kinase kinase 4 MAP3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase MET MET proto-oncogene, receptor tyrosine kinase MRE11A MRE11 meiotic recombination 11 homolog A (S. cerevisiae) NBN nibrin NF1 neurofibromin 1 NOTCH1 notch 1 NOTCH2 notch 2 NOTCH3 notch 3 NOTCH4 notch 4 NQO1 NAD(P)H dehydrogenase, quinone 1 PALB2 partner and localizer of BRCA2 PDGFRA platelet-derived growth factor receptor, alpha polypeptide PGR progesterone receptor PIK3CA phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha PTCH1 patched 1 PTEN phosphatase and tensin homolog PTENP1 phosphatase and tensin homolog pseudogene 1 (functional) RAD50 RAD50 homolog (S. cerevisiae) RAD51 RAD51 recombinase RAD51B RAD51 paralog B RAD51C RAD51 paralog C RAD51D RAD51 paralog D RB1 retinoblastoma 1 SMO smoothened, frizzled class receptor SOD2 superoxide dismutase 2, mitochondrial STK11 serine/threonine kinase 11 TNF tumor necrosis factor TP53 tumor protein p53 ACTB Actin, beta B2M Beta-2-microglobulin GAPD Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |