A ready-to-use Human T-Cell Development/Differentiation PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against T-cell development and differentiation genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: ATF5 activating transcription factor 5 BACH2 BTB and CNC homology 1, basic leucine zipper transcription factor 2 BATF basic leucine zipper transcription factor, ATF-like BCL11B B-cell CLL/lymphoma 11B (zinc finger protein) BCL6 B-cell CLL/lymphoma 6 BTK Bruton agammaglobulinemia tyrosine kinase CBFA2T3 core-binding factor, runt domain, alpha subunit 2; translocated to, 3 CCR7 chemokine (C-C motif) receptor 7 CD247 CD247 molecule CD27 CD27 molecule CD3D CD3d molecule, delta (CD3-TCR complex) CD3E CD3e molecule, epsilon (CD3-TCR complex) CD3G CD3g molecule, gamma (CD3-TCR complex) CD4 CD4 molecule CD44 CD44 molecule (Indian blood group) CD69 CD69 molecule CD8A CD8a molecule CD8B CD8b molecule CSF1R colony stimulating factor 1 receptor DCLRE1C DNA cross-link repair 1C EOMES eomesodermin EZH2 enhancer of zeste 2 polycomb repressive complex 2 subunit FOXP3 forkhead box P3 GATA3 GATA binding protein 3 GFI1 growth factor independent 1 transcription repressor IFNG interferon, gamma IKZF1 IKAROS family zinc finger 1 (Ikaros) IL15 interleukin 15 IL15RA interleukin 15 receptor, alpha IL17A interleukin 17A IL2 interleukin 2 IL21 interleukin 21 IL21R interleukin 21 receptor IL2RA interleukin 2 receptor, alpha IL2RB interleukin 2 receptor, beta IL2RG interleukin 2 receptor, gamma IL4R interleukin 4 receptor IL7 interleukin 7 IL7R interleukin 7 receptor IL9 interleukin 9 IL9R interleukin 9 receptor IRF4 interferon regulatory factor 4 ITK IL2-inducible T-cell kinase LAT linker for activation of T cells LCK LCK proto-oncogene, Src family tyrosine kinase LCP2 lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) LIG4 ligase IV, DNA, ATP-dependent LYN LYN proto-oncogene, Src family tyrosine kinase MAPK1 mitogen-activated protein kinase 1 MAPK3 mitogen-activated protein kinase 3 MAPK8 mitogen-activated protein kinase 8 MAPK9 mitogen-activated protein kinase 9 MYB v-myb avian myeloblastosis viral oncogene homolog NFATC2 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 NFE2 nuclear factor, erythroid 2 NOTCH1 notch 1 NOTCH3 notch 3 NR4A2 nuclear receptor subfamily 4, group A, member 2 PHF7 PHD finger protein 7 PLCG1 phospholipase C, gamma 1 POU2F1 POU class 2 homeobox 1 POU5F1 POU class 5 homeobox 1 PRDM1 PR domain containing 1, with ZNF domain PRKDC protein kinase, DNA-activated, catalytic polypeptide PTCRA pre T-cell antigen receptor alpha RAG1 recombination activating gene 1 RAG2 recombination activating gene 2 RASGRP1 RAS guanyl releasing protein 1 (calcium and DAG- regulated) REL v-rel avian reticuloendotheliosis viral oncogene homolog RELA v-rel avian reticuloendotheliosis viral oncogene homolog A RUNX1 runt-related transcription factor 1 RUNX3 runt-related transcription factor 3 SH2D1A SH2 domain containing 1A SOS1 son of sevenless homolog 1 (Drosophila) STAT1 signal transducer and activator of transcription 1, 91kDa STAT3 signal transducer and activator of transcription 3 (acute-phase response factor) STAT5A signal transducer and activator of transcription 5A STAT5B signal transducer and activator of transcription 5B SYK spleen tyrosine kinase TBX21 T-box 21 TCF7 transcription factor 7 (T-cell specific, HMG-box) TEC Tecprotein tyrosine kinase THY1 Thy-1 cell surface antigen TOX thymocyte selection-associated high mobility group box ZAP70 zeta-chain (TCR) associated protein kinase 70kDa ZBTB16 zinc finger and BTB domain containing 16 ZBTB25 zinc finger and BTB domain containing 25 ZBTB7B zinc finger and BTB domain containing 7B ACTB Actin, beta B2M Beta-2-microglobulin GAPDH Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |