Human T-Cell Development and Differentiation Primer Library

Contains 88 primer sets directed against T-cell development and differentiation genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ATF5 activating transcription factor 5

BACH2 BTB and CNC homology 1, basic leucine zipper transcription factor 2

BATF basic leucine zipper transcription factor, ATF-like

BCL11B B-cell CLL/lymphoma 11B (zinc finger protein)

BCL6 B-cell CLL/lymphoma 6

BTK Bruton agammaglobulinemia tyrosine kinase

CBFA2T3 core-binding factor, runt domain, alpha subunit 2; translocated to, 3

CCR7 chemokine (C-C motif) receptor 7

CD247 CD247 molecule

CD27 CD27 molecule

CD3D CD3d molecule, delta (CD3-TCR complex)

CD3E CD3e molecule, epsilon (CD3-TCR complex)

CD3G CD3g molecule, gamma (CD3-TCR complex)

CD4 CD4 molecule

CD44 CD44 molecule (Indian blood group)

CD69 CD69 molecule

CD8A CD8a molecule

CD8B CD8b molecule

CSF1R colony stimulating factor 1 receptor

DCLRE1C DNA cross-link repair 1C

EOMES eomesodermin

EZH2 enhancer of zeste 2 polycomb repressive complex 2 subunit

FOXP3 forkhead box P3

GATA3 GATA binding protein 3

GFI1 growth factor independent 1 transcription repressor

IFNG interferon, gamma

IKZF1 IKAROS family zinc finger 1 (Ikaros)

IL15 interleukin 15

IL15RA interleukin 15 receptor, alpha

IL17A interleukin 17A

IL2 interleukin 2

IL21 interleukin 21

IL21R interleukin 21 receptor

IL2RA interleukin 2 receptor, alpha

IL2RB interleukin 2 receptor, beta

IL2RG interleukin 2 receptor, gamma

IL4R interleukin 4 receptor

IL7 interleukin 7

IL7R interleukin 7 receptor

IL9 interleukin 9

IL9R interleukin 9 receptor

IRF4 interferon regulatory factor 4

ITK IL2-inducible T-cell kinase

LAT linker for activation of T cells

LCK LCK proto-oncogene, Src family tyrosine kinase

LCP2 lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)

LIG4 ligase IV, DNA, ATP-dependent

LYN LYN proto-oncogene, Src family tyrosine kinase

MAPK1 mitogen-activated protein kinase 1

MAPK3 mitogen-activated protein kinase 3

MAPK8 mitogen-activated protein kinase 8

MAPK9 mitogen-activated protein kinase 9

MYB v-myb avian myeloblastosis viral oncogene homolog

NFATC2 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2

NFE2 nuclear factor, erythroid 2

NOTCH1 notch 1

NOTCH3 notch 3

NR4A2 nuclear receptor subfamily 4, group A, member 2

PHF7 PHD finger protein 7

PLCG1 phospholipase C, gamma 1

POU2F1 POU class 2 homeobox 1

POU5F1 POU class 5 homeobox 1

PRDM1 PR domain containing 1, with ZNF domain

PRKDC protein kinase, DNA-activated, catalytic polypeptide

PTCRA pre T-cell antigen receptor alpha

RAG1 recombination activating gene 1

RAG2 recombination activating gene 2

RASGRP1 RAS guanyl releasing protein 1 (calcium and DAG- regulated)

REL v-rel avian reticuloendotheliosis viral oncogene homolog

RELA v-rel avian reticuloendotheliosis viral oncogene homolog A

RUNX1 runt-related transcription factor 1

RUNX3 runt-related transcription factor 3

SH2D1A SH2 domain containing 1A

SOS1 son of sevenless homolog 1 (Drosophila)

STAT1 signal transducer and activator of transcription 1, 91kDa

STAT3 signal transducer and activator of transcription 3 (acute-phase response factor)

STAT5A signal transducer and activator of transcription 5A

STAT5B signal transducer and activator of transcription 5B

SYK spleen tyrosine kinase

TBX21 T-box 21

TCF7 transcription factor 7 (T-cell specific, HMG-box)

TEC Tec protein tyrosine kinase

THY1 Thy-1 cell surface antigen

TOX thymocyte selection-associated high mobility group box

ZAP70 zeta-chain (TCR) associated protein kinase 70kDa

ZBTB16 zinc finger and BTB domain containing 16

ZBTB25 zinc finger and BTB domain containing 25

ZBTB7B zinc finger and BTB domain containing 7B

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a