Statin Signaling

Statins represent a class of lipid lowering drugs developed for the treatment of cardiovascular disease. Inhibition of hydroxymethylglutaryl coenzyme A reductase (HMGCR), a key enzyme of the mevalonate pathway, by statin drugs results in pleiotropic effects. In addition to a positive effect in reducing serum cholesterol, statins have proven to have some activity as anticancer agents and may be useful for the treatment of other diseases, likely through the effects of statins on several other cellular pathways including MAPK, NFKB, AKT, PPAR, apoptosis and inflammatory signaling. This Statin signaling PCR primer library contains primer sets directed not only at the genes regulating the mevalonate pathway, but include many of the downstream effector molecules that are up- and down-regulated following statin exposure.

A ready-to-use Human Statin Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against statin signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ABCA1 ATP-binding cassette, sub-family A (ABC1), member 1

ABCG5 ATP-binding cassette, sub-family G (WHITE), member 5

ABCG8 ATP-binding cassette, sub-family G (WHITE), member 8

ACAT2 Acetyl-CoA acetyltransferase 2

AKT1 V-akt murine thymoma viral oncogene homolog 1

APOA1 Apolipoprotein A-I

APOA2 Apolipoprotein A-II

APOA4 Apolipoprotein A-IV

APOA5 Apolipoprotein A-V

APOB Apolipoprotein B (including Ag(x) antigen)

APOC1 Apolipoprotein C-I

APOC2 Apolipoprotein C-II

APOC3 Apolipoprotein C-III

APOE Apolipoprotein E

BAX BCL2-associated X protein

BCL2 B-cell CLL/lymphoma 2

BCL2L1 BCL2-like 1

BDNF Brain-derived neurotrophic factor

CASP3 Caspase 3, apoptosis-related cysteine peptidase

CASP7 Caspase 7, apoptosis-related cysteine peptidase

CASP9 Caspase 9, apoptosis-related cysteine peptidase

CAV1 Caveolin 1, caveolae protein, 22kDa

CD40 CD40 molecule, TNF receptor superfamily member 5

CDC42 Cell division cycle 42 (GTP binding protein, 25kDa)

CETP Cholesteryl ester transfer protein, plasma

CYP3A4 Cytochrome P450, family 3, subfamily A, polypeptide 4

CYP3A5 Cytochrome P450, family 3, subfamily A, polypeptide 5

CYP7A1 Cytochrome P450, family 7, subfamily A, polypeptide 1

DGAT1 Diacylglycerol O-acyltransferase 1

ELK1 ELK1, member of ETS oncogene family

FASN Fatty acid synthase

FDFT1 Farnesyl-diphosphate farnesyltransferase 1

GSK3B Glycogen synthase kinase 3 beta

HMGCR 3-hydroxy-3-methylglutaryl-CoA reductase

HMGCS1 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble)

IFNG Interferon, gamma

IKBKB Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta

IKBKE Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon

IKBKG Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma

IL1B Interleukin 1, beta

IL2 Interleukin 2

IL5 Interleukin 5 (colony-stimulating factor, eosinophil)

IL6 Interleukin 6 (interferon, beta 2)

JUN Jun proto-oncogene

KLF2 Kruppel-like factor 2 (lung)

LCAT Lecithin-cholesterol acyltransferase

LDLR Low density lipoprotein receptor

LIPC Lipase, hepatic

LPL Lipoprotein lipase

LRP1 Low density lipoprotein receptor-related protein 1

LSS Lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)

MAPK1 Mitogen-activated protein kinase 1

MAPK10 Mitogen-activated protein kinase 10

MAPK14 Mitogen-activated protein kinase 14

MAPK3 Mitogen-activated protein kinase 3

MDM2 Mdm2 p53 binding protein homolog (mouse)

MTOR Mechanistic target of rapamycin (serine/threonine kinase)

MTTP Microsomal triglyceride transfer protein

NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NGF Nerve growth factor (beta polypeptide)

NOS3 Nitric oxide synthase 3 (endothelial cell)

PDIA2 Protein disulfide isomerase family A, member 2

PDK1 Pyruvate dehydrogenase kinase, isozyme 1

PLTP Phospholipid transfer protein

PPARA Peroxisome proliferator activated receptor alpha

PPARD Peroxisome proliferator-activated receptor delta

PPARG Peroxisome proliferator-activated receptor gamma

PPARGC1A Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha

PPARGC1B Peroxisome proliferator-activated receptor gamma, coactivator 1 beta

PTEN Phosphatase and tensin homolog

PTGS2 Prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

RAC1 Ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

REL V-rel reticuloendotheliosis viral oncogene homolog (avian)

RELA V-rel reticuloendotheliosis viral oncogene homolog A (avian)

ROCK1 Rho-associated, coiled-coil containing protein kinase 1

SCARB1 Scavenger receptor class B, member 1

SCD Stearoyl-CoA desaturase (delta-9-desaturase)

SIRT1 Sirtuin 1

SMAD3 SMAD family member 3

SMAD4 SMAD family member 4

SOAT1 Sterol O-acyltransferase 1

SQLE Squalene epoxidase

SREBf1 Sterol regulatory element binding transcription factor 1

SREBf2 Sterol regulatory element binding transcription factor 2

TGFB1 Transforming growth factor, beta 1

TLR4 Toll-like receptor 4

TNF Tumor necrosis factor

VEGFA Vascular endothelial growth factor A

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
HSTAT-I