Nuclear factor kappa B (NF-kappaB) is a family of transcription factors (RELA, RELB, REL, NFKB1 and NFKB2) that control inflammation, immunity, cell survival and proliferation. In the canonical pathway, signals from TNF, IL-1, Toll-like receptors and antigen receptors activate the IKK complex (IKBKB, CHUK, IKBKG/NEMO), which phosphorylates IkappaB proteins and targets them for degradation, freeing NF-kappaB to enter the nucleus. The non-canonical pathway, activated by receptors such as CD40, LTBR and BAFFR, works through NIK and IKK-alpha to process NFKB2. NF-kappaB target genes include cytokines, chemokines, adhesion molecules and anti-apoptotic proteins.
A ready-to-use Human NFKappaB Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against NFKappaB signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: AKT1 v-akt murine thymoma viral oncogene homolog 1 AKT2 v-akt murine thymoma viral oncogene homolog 2 AKT3 v-akt murine thymoma viral oncogene homolog 3 BCL2A1 BCL2-related protein A1 BCL2L1 BCL2-like 1 BIRC2 baculoviral IAP repeat containing 2 BIRC3 baculoviral IAP repeat containing 3 BIRC4 baculoviral IAP repeat containing 4 BIRC5 baculoviral IAP repeat containing 5 CARD10 caspase recruitment domain family, member 10 CARD11 caspase recruitment domain family, member 11 CASP10 caspase 10, apoptosis-related cysteine peptidase CASP8 caspase 8, apoptosis-related cysteine peptidase CCL2 chemokine (C-C motif) ligand 2 CCL3 chemokine (C-C motif) ligand 3 CCL4 chemokine (C-C motif) ligand 4 CCL5 chemokine (C-C motif) ligand 5 CD40 CD40 molecule, TNF receptor superfamily member 5 CD80 CD80 molecule CD86 CD86 molecule CFLAR CASP8 and FADD-like apoptosis regulator CHUK conserved helix-loop-helix ubiquitous kinase FAS Fas cell surface death receptor FASLG Fas ligand (TNF superfamily, member 6) ICAM1 intercellular adhesion molecule 1 IKBKB inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta IKBKE inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon IKBKG inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma IL10 interleukin 10 IL12B interleukin 12B IL18 interleukin 18 IL1B interleukin 1, beta IL6 interleukin 6 IL8 interleukin 8 MAP2K1 mitogen-activated protein kinase kinase 1 MAP2K2 mitogen-activated protein kinase kinase 2 MAP2K3 mitogen-activated protein kinase kinase 3 MAP2K4 mitogen-activated protein kinase kinase 4 MAP2K6 mitogen-activated protein kinase kinase 6 MAP2K7 mitogen-activated protein kinase kinase 7 MAP3K14 mitogen-activated protein kinase kinase kinase 14 MAP3K7 mitogen-activated protein kinase kinase kinase 7 MAPK1 mitogen-activated protein kinase 1 MAPK10 mitogen-activated protein kinase 10 MAPK14 mitogen-activated protein kinase 14 MAPK3 mitogen-activated protein kinase 3 MAPK8 mitogen-activated protein kinase 8 MAPK9 mitogen-activated protein kinase 9 MAPKAPK2 mitogen-activated protein kinase-activated protein kinase 2 MAPKAPK2 mitogen-activated protein kinase-activated protein kinase 2 MAPKAPK3 mitogen-activated protein kinase-activated protein kinase 3 MAPKAPK3 mitogen-activated protein kinase-activated protein kinase 3 MAPKAPK5 mitogen-activated protein kinase-activated protein kinase 5 NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 NFKB2 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha NFKBIB nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta NFKBIE nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon NFKBIZ nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta NLRC4 NLR family, CARD domain containing 4 NOD1 nucleotide-binding oligomerization domain containing 1 NOD2 nucleotide-binding oligomerization domain containing 2 REL v-rel avian reticuloendotheliosis viral oncogene homolog RELA v-rel avian reticuloendotheliosis viral oncogene homolog A RELB v-rel avian reticuloendotheliosis viral oncogene homolog B RIPK1 receptor (TNFRSF)-interacting serine-threonine kinase 1 RIPK2 receptor-interacting serine-threonine kinase 2 SELE selectin E TAB1 TGF-beta activated kinase 1/MAP3K7 binding protein 1 TAB2 TGF-beta activated kinase 1/MAP3K7 binding protein 2 TAB3 TGF-beta activated kinase 1/MAP3K7 binding protein 3 TNF tumor necrosis factor TNFAIP3 tumor necrosis factor, alpha-induced protein 3 TNFRSF11A tumor necrosis factor receptor superfamily, member 11a, NFKB activator TNFRSF13B tumor necrosis factor receptor superfamily, member 13B TNFRSF14 tumor necrosis factor receptor superfamily, member 14 TNFRSF1A tumor necrosis factor receptor superfamily, member 1A TNFRSF1B tumor necrosis factor receptor superfamily, member 1B TNFRSF25 tumor necrosis factor receptor superfamily, member 25 TNFRSF8 tumor necrosis factor receptor superfamily, member 8 TNFSF13B tumor necrosis factor (ligand) superfamily, member 13b TRADD TNFRSF1A-associated via death domain TRAF1 TNF receptor-associated factor 1 TRAF2 TNF receptor-associated factor 2 TRAF3 TNF receptor-associated factor 3 TRAF4 TNF receptor-associated factor 4 TRAF6 TNF receptor-associated factor 6, E3 ubiquitin protein ligase VCAM1 vascular cell adhesion molecule 1 ACTB Actin, beta B2M Beta-2-microglobulin GAPD Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |