Mitogen activated protein kinase (MAPK) pathways are three tiered kinase cascades in which a MAP3K activates a MAP2K, which then activates a MAPK. The main branches are the ERK1/2 pathway (RAF-MEK-ERK), activated mainly by growth factors through RAS, and the JNK and p38 pathways, activated mainly by stress and inflammatory cytokines. MAPKs phosphorylate transcription factors such as ELK1, JUN, ATF2 and MYC to control proliferation, differentiation, inflammation and apoptosis. Mutations in RAS and BRAF make the MAPK pathway one of the most frequently activated pathways in cancer.
A ready-to-use Human MAP Kinase Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against MAP kinase signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: ARAF v-raf murine sarcoma 3611 viral oncogene homolog ATF2 activating transcription factor 2 BRAF v-raf murine sarcoma viral oncogene homolog B1 CCND1 cyclin D1 CCND2 cyclin D2 CCNE1 cyclin E1 CDC42 cell division cycle 42 (GTP binding protein, 25kDa) CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1) CDKN1C cyclin-dependent kinase inhibitor 1C (p57, Kip2) CHUK conserved helix-loop-helix ubiquitous kinase COL1A1 collagen, type I, alpha 1 CREB1 cAMP responsive element binding protein 1 CREBBP CREB binding protein DUSP1 dual specificity phosphatase 1 DUSP10 dual specificity phosphatase 10 DUSP16 dual specificity phosphatase 16 DUSP2 dual specificity phosphatase 2 DUSP4 dual specificity phosphatase 4 DUSP5 dual specificity phosphatase 5 DUSP6 dual specificity phosphatase 6 DUSP7 dual specificity phosphatase 7 DUSP8 dual specificity phosphatase 8 DUSP9 dual specificity phosphatase 9 FOS FBJ murine osteosarcoma viral oncogene homolog GRB2 growth factor receptor-bound protein 2 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog IGF1 insulin-like growth factor 1 (somatomedin C) KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog MAP2K1 mitogen-activated protein kinase kinase 1 MAP2K2 mitogen-activated protein kinase kinase 2 MAP2K3 mitogen-activated protein kinase kinase 3 MAP2K4 mitogen-activated protein kinase kinase 4 MAP2K5 mitogen-activated protein kinase kinase 5 MAP2K6 mitogen-activated protein kinase kinase 6 MAP2K7 mitogen-activated protein kinase kinase 7 MAP3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase MAP3K10 mitogen-activated protein kinase kinase kinase 10 MAP3K11 mitogen-activated protein kinase kinase kinase 11 MAP3K2 mitogen-activated protein kinase kinase kinase 2 MAP3K3 mitogen-activated protein kinase kinase kinase 3 MAP3K4 mitogen-activated protein kinase kinase kinase 4 MAP3K5 mitogen-activated protein kinase kinase kinase 5 MAP3K6 mitogen-activated protein kinase kinase kinase 6 MAP3K7 mitogen-activated protein kinase kinase kinase 7 MAP3K8 mitogen-activated protein kinase kinase kinase 8 MAP3K9 mitogen-activated protein kinase kinase kinase 9 MAP4K1 mitogen-activated protein kinase kinase kinase kinase 1 MAP4K2 mitogen-activated protein kinase kinase kinase kinase 2 MAPK1 mitogen-activated protein kinase 1 MAPK10 mitogen-activated protein kinase 10 MAPK11 mitogen-activated protein kinase 11 MAPK12 mitogen-activated protein kinase 12 MAPK13 mitogen-activated protein kinase 13 MAPK14 mitogen-activated protein kinase 14 MAPK15 mitogen-activated protein kinase 15 MAPK3 mitogen-activated protein kinase 3 MAPK4 mitogen-activated protein kinase 4 MAPK6 mitogen-activated protein kinase 6 MAPK7 mitogen-activated protein kinase 7 MAPK8 mitogen-activated protein kinase 8 MAPK8IP1 mitogen-activated protein kinase 8 interacting protein 1 MAPK8IP2 mitogen-activated protein kinase 8 interacting protein 2 MAPK9 mitogen-activated protein kinase 9 MAPKAPK2 mitogen-activated protein kinase-activated protein kinase 2 MAPKAPK3 mitogen-activated protein kinase-activated protein kinase 3 MAPKAPK5 mitogen-activated protein kinase-activated protein kinase 5 MEF2A myocyte enhancer factor 2A MEF2C myocyte enhancer factor 2C MYC V-myc myelocytomatosis viral oncogene homolog (avian) NLK nemo-like kinase NRAS neuroblastoma RAS viral (v-ras) oncogene homolog PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 PAK2 p21 protein (Cdc42/Rac)-activated kinase 2 PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 PAK4 p21 protein (Cdc42/Rac)-activated kinase 4 PTK2B protein tyrosine kinase 2 beta RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) RAF1 v-raf-1 murine leukemia viral oncogene homolog 1 RELA v-rel reticuloendotheliosis viral oncogene homolog A (avian) RPS6KA1 ribosomal protein S6 kinase, 90kDa, polypeptide 1 RPS6KA2 ribosomal protein S6 kinase, 90kDa, polypeptide 2 RPS6KA3 ribosomal protein S6 kinase, 90kDa, polypeptide 3 RPS6KA4 ribosomal protein S6 kinase, 90kDa, polypeptide 4 RPS6KA5 ribosomal protein S6 kinase, 90kDa, polypeptide 5 RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 RPS6KB2 ribosomal protein S6 kinase, 70kDa, polypeptide 2 SHC1 SHC (Src homology 2 domain containing) transforming protein 1 TP53 tumor protein p53 ACTB Actin, beta B2M Beta-2-microglobulin GAPDH Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |
A ready-to-use Mouse MAP Kinase Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against MAP kinase signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: ARAF v-raf murine sarcoma 3611 viral oncogene homolog ATF2 activating transcription factor 2 BRAF v-raf murine sarcoma viral oncogene homolog B1 CCND1 cyclin D1 CCND2 cyclin D2 CCNE1 cyclin E1 CDC42 cell division cycle 42 (GTP binding protein, 25kDa) CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1) CDKN1C cyclin-dependent kinase inhibitor 1C (p57, Kip2) CHUK conserved helix-loop-helix ubiquitous kinase COL1A1 collagen, type I, alpha 1 CREB1 cAMP responsive element binding protein 1 CREBBP CREB binding protein DUSP1 dual specificity phosphatase 1 DUSP10 dual specificity phosphatase 10 DUSP16 dual specificity phosphatase 16 DUSP2 dual specificity phosphatase 2 DUSP4 dual specificity phosphatase 4 DUSP5 dual specificity phosphatase 5 DUSP6 dual specificity phosphatase 6 DUSP7 dual specificity phosphatase 7 DUSP8 dual specificity phosphatase 8 DUSP9 dual specificity phosphatase 9 FOS FBJ murine osteosarcoma viral oncogene homolog GRB2 growth factor receptor-bound protein 2 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog IGF1 insulin-like growth factor 1 (somatomedin C) KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog MAP2K1 mitogen-activated protein kinase kinase 1 MAP2K2 mitogen-activated protein kinase kinase 2 MAP2K3 mitogen-activated protein kinase kinase 3 MAP2K4 mitogen-activated protein kinase kinase 4 MAP2K5 mitogen-activated protein kinase kinase 5 MAP2K6 mitogen-activated protein kinase kinase 6 MAP2K7 mitogen-activated protein kinase kinase 7 MAP3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase MAP3K10 mitogen-activated protein kinase kinase kinase 10 MAP3K11 mitogen-activated protein kinase kinase kinase 11 MAP3K2 mitogen-activated protein kinase kinase kinase 2 MAP3K3 mitogen-activated protein kinase kinase kinase 3 MAP3K4 mitogen-activated protein kinase kinase kinase 4 MAP3K5 mitogen-activated protein kinase kinase kinase 5 MAP3K6 mitogen-activated protein kinase kinase kinase 6 MAP3K7 mitogen-activated protein kinase kinase kinase 7 MAP3K8 mitogen-activated protein kinase kinase kinase 8 MAP3K9 mitogen-activated protein kinase kinase kinase 9 MAP4K1 mitogen-activated protein kinase kinase kinase kinase 1 MAP4K2 mitogen-activated protein kinase kinase kinase kinase 2 MAPK1 mitogen-activated protein kinase 1 MAPK10 mitogen-activated protein kinase 10 MAPK11 mitogen-activated protein kinase 11 MAPK12 mitogen-activated protein kinase 12 MAPK13 mitogen-activated protein kinase 13 MAPK14 mitogen-activated protein kinase 14 MAPK15 mitogen-activated protein kinase 15 MAPK3 mitogen-activated protein kinase 3 MAPK4 mitogen-activated protein kinase 4 MAPK6 mitogen-activated protein kinase 6 MAPK7 mitogen-activated protein kinase 7 MAPK8 mitogen-activated protein kinase 8 MAPK8IP1 mitogen-activated protein kinase 8 interacting protein 1 MAPK8IP2 mitogen-activated protein kinase 8 interacting protein 2 MAPK9 mitogen-activated protein kinase 9 MAPKAPK2 mitogen-activated protein kinase-activated protein kinase 2 MAPKAPK3 mitogen-activated protein kinase-activated protein kinase 3 MAPKAPK5 mitogen-activated protein kinase-activated protein kinase 5 MEF2A myocyte enhancer factor 2A MEF2C myocyte enhancer factor 2C MYC V-myc myelocytomatosis viral oncogene homolog (avian) NLK nemo-like kinase NRAS neuroblastoma RAS viral (v-ras) oncogene homolog PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 PAK2 p21 protein (Cdc42/Rac)-activated kinase 2 PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 PAK4 p21 protein (Cdc42/Rac)-activated kinase 4 PTK2B protein tyrosine kinase 2 beta RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) RAF1 v-raf-1 murine leukemia viral oncogene homolog 1 RELA v-rel reticuloendotheliosis viral oncogene homolog A (avian) RPS6KA1 ribosomal protein S6 kinase, 90kDa, polypeptide 1 RPS6KA2 ribosomal protein S6 kinase, 90kDa, polypeptide 2 RPS6KA3 ribosomal protein S6 kinase, 90kDa, polypeptide 3 RPS6KA4 ribosomal protein S6 kinase, 90kDa, polypeptide 4 RPS6KA5 ribosomal protein S6 kinase, 90kDa, polypeptide 5 RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 RPS6KB2 ribosomal protein S6 kinase, 70kDa, polypeptide 2 SHC1 SHC (Src homology 2 domain containing) transforming protein 1 TP53 tumor protein p53 ACTB Actin, beta B2M Beta-2-microglobulin GAPDH Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |