Human T-Cell Anergy/Immune Tolerance Primer Library

Contains 88 primer sets directed against T-cell anergy and immune tolerance genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

BTLA B and T lymphocyte associated

CBLB Cbl proto-oncogene B, E3 ubiquitin protein ligase

CD27 CD27 molecule

CD274 CD274 molecule

CD28 CD28 molecule

CD3E CD3e molecule, epsilon (CD3-TCR complex)

CD40 CD40 molecule, TNF receptor superfamily member 5

CD40LG CD40 ligand

CD80 CD80 molecule

CD86 CD86 molecule

CDK2 cyclin-dependent kinase 2

CDK4 cyclin-dependent kinase 4

CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1)

CREB1 cAMP responsive element binding protein 1

CREM cAMP responsive element modulator

CSF1 colony stimulating factor 1 (macrophage)

CSF2 colony stimulating factor 2 (granulocyte-macrophage)

CTLA4 cytotoxic T-lymphocyte-associated protein 4

CXCR3 chemokine (C-X-C motif) receptor 3

CXCR4 chemokine (C-X-C motif) receptor 4

DGKA diacylglycerol kinase, alpha 80kDa

DGKG diacylglycerol kinase, gamma 90kDa

DGKZ diacylglycerol kinase, zeta

EGR1 early growth response 1

EGR2 early growth response 2

EGR3 early growth response 3

EOMES eomesodermin

FAS Fas cell surface death receptor

FASLG Fas ligand (TNF superfamily, member 6)

FOS FBJ murine osteosarcoma viral oncogene homolog

FOXP3 forkhead box P3

GATA3 GATA binding protein 3

GRB2 growth factor receptor-bound protein 2

GZMB granzyme B (granzyme 2, cytotoxic T-lymphocyte- associated serine esterase 1)

HACE1 HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1

HDAC1 histone deacetylase 1

HDAC9 histone deacetylase 9

ICAM1 intercellular adhesion molecule 1

ICOS inducible T-cell co-stimulator

IFNG interferon, gamma

IL10 interleukin 10

IL13 interleukin 13

IL15 interleukin 15

IL15RA interleukin 15 receptor, alpha

IL17A interleukin 17A

IL1A interleukin 1, alpha

IL1R1 interleukin 1 receptor, type I

IL1R2 interleukin 1 receptor, type II

IL2 interleukin 2

IL21 interleukin 21

IL21R interleukin 21 receptor

IL2RA interleukin 2 receptor, alpha

IL2RB interleukin 2 receptor, beta

IL3 interleukin 3

IL4 interleukin 4

IL5 interleukin 5

IL6 interleukin 6

IL7R interleukin 7 receptor

IRF4 interferon regulatory factor 4

ITCH itchy E3 ubiquitin protein ligase

JAK1 Janus kinase 1

JAK3 Janus kinase 3

JUN jun proto-oncogene

LGALS3 lectin, galactoside-binding, soluble, 3

LTA lymphotoxin alpha

MEF2A myocyte enhancer factor 2A

NFATC1 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

NFATC2 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2

NFATC3 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3

NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NOTCH1 notch 1

PDCD1 programmed cell death 1

PLCG1 phospholipase C, gamma 1

PRKCG protein kinase C, gamma

PTGER2 prostaglandin E receptor 2 (subtype EP2), 53kDa

PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

RAF1 Raf-1 proto-oncogene, serine/threonine kinase

RAPGEF3 Rap guanine nucleotide exchange factor (GEF) 3

RASGRP1 RAS guanyl releasing protein 1 (calcium and DAG- regulated)

RNF128 ring finger protein 128, E3 ubiquitin protein ligase

SELL selectin L

SOS1 son of sevenless homolog 1 (Drosophila)

STAT3 signal transducer and activator of transcription 3 (acute-phase response factor)

TGFB1 transforming growth factor, beta 1

TNFRSF10A tumor necrosis factor receptor superfamily, member 10a

TNFSF10 tumor necrosis factor (ligand) superfamily, member 10

TRAF6 TNF receptor-associated factor 6, E3 ubiquitin protein ligase

ZAP70 zeta-chain (TCR) associated protein kinase 70kDa

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a