Human Ras Signaling Primer Library

Contains 88 primer sets directed against Ras signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ACTA1 actin, alpha 1, skeletal muscle

ACTA2 actin, alpha 2, smooth muscle, aorta

ACTG1 actin, gamma 1

ACTG2 actin, gamma 2, smooth muscle, enteric

ARAF v-raf murine sarcoma 3611 viral oncogene homolog

ARHGAP5 Rho GTPase activating protein 5

ARHGEF1 Rho guanine nucleotide exchange factor (GEF) 1

ARHGEF11 Rho guanine nucleotide exchange factor (GEF) 11

ARHGEF2 Rho/Rac guanine nucleotide exchange factor (GEF) 2

ARHGEF6 Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6

ARHGEF7 Rho guanine nucleotide exchange factor (GEF) 7

ATF2 activating transcription factor 2

BRAF v-raf murine sarcoma viral oncogene homolog B1

CD247 CD247 molecule

CD3E CD3e molecule, epsilon (CD3-TCR complex)

CDC42 cell division cycle 42 (GTP binding protein, 25kDa)

EGF epidermal growth factor

EGFR epidermal growth factor receptor

ELK1 ELK1, member of ETS oncogene family

ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

FGF1 fibroblast growth factor 1 (acidic)

FGF2 fibroblast growth factor 2 (basic)

FOS FBJ murine osteosarcoma viral oncogene homolog

GPLD1 glycosylphosphatidylinositol specific phospholipase D1

GRB2 growth factor receptor-bound protein 2

HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog

IGF1R insulin-like growth factor 1 receptor

ITGA2B integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)

ITGAV integrin, alpha V

ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)

ITGB2 integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)

ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)

JUN jun proto-oncogene

KDR kinase insert domain receptor (a type III receptor tyrosine kinase)

KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

LCK lymphocyte-specific protein tyrosine kinase

MAP2K1 mitogen-activated protein kinase kinase 1

MAP2K2 mitogen-activated protein kinase kinase 2

MAP2K3 mitogen-activated protein kinase kinase 3

MAP2K4 mitogen-activated protein kinase kinase 4

MAP2K6 mitogen-activated protein kinase kinase 6

MAP2K7 mitogen-activated protein kinase kinase 7

MAP3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase

MAPK1 mitogen-activated protein kinase 1

MAPK10 mitogen-activated protein kinase 10

MAPK12 mitogen-activated protein kinase 12

MAPK3 mitogen-activated protein kinase 3

MAPK6 mitogen-activated protein kinase 6

MAPK7 mitogen-activated protein kinase 7

MAPK8 mitogen-activated protein kinase 8

MAPK9 mitogen-activated protein kinase 9

NRAS neuroblastoma RAS viral (v-ras) oncogene homolog

PAK1 p21 protein (Cdc42/Rac)-activated kinase 1

PAK2 p21 protein (Cdc42/Rac)-activated kinase 2

PAK3 p21 protein (Cdc42/Rac)-activated kinase 3

PAK4 p21 protein (Cdc42/Rac)-activated kinase 4

PAK7 p21 protein (Cdc42/Rac)-activated kinase 7

PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide

PIK3CB phosphoinositide-3-kinase, catalytic, beta polypeptide

PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide

PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

PIK3R2 phosphoinositide-3-kinase, regulatory subunit 2 (beta)

PLCE1 phospholipase C, epsilon 1

PLD1 phospholipase D1, phosphatidylcholine-specific

PLD2 phospholipase D2

PLD3 phospholipase D family, member 3

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RAC2 ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)

RAC3 ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)

RAF1 v-raf-1 murine leukemia viral oncogene homolog 1

RALA v-ral simian leukemia viral oncogene homolog A (ras related)

RALB v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)

RALBP1 ralA binding protein 1

RALGDS ral guanine nucleotide dissociation stimulator

RAP1A RAP1A, member of RAS oncogene family

RASA1 RAS p21 protein activator (GTPase activating protein) 1

RHOA ras homolog family member A

RHOB ras homolog family member B

RHOC ras homolog family member C

RHOG ras homolog family member G

RRAS related RAS viral (r-ras) oncogene homolog

RRAS2 related RAS viral (r-ras) oncogene homolog 2

SHC1 SHC (Src homology 2 domain containing) transforming protein 1

SHC3 SHC (Src homology 2 domain containing) transforming protein 3

SOS1 son of sevenless homolog 1

SOS2 son of sevenless homolog 2

VEGFA vascular endothelial growth factor A

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a