Human Melanoma Primer Library

Contains 88 primer sets directed against melanoma associated genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul; - 10 uM;)

Perform up to 100 PCR arrays (based on 20 ul; assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

AKT1 v-akt murine thymoma viral oncogene homolog 1

AKT2 v-akt murine thymoma viral oncogene homolog 2

AKT3 v-akt murine thymoma viral oncogene homolog 3

ARID2 AT rich interactive domain 2 (ARID, RFX-like)

ATF2 activating transcription factor 2

BAP1 BRCA1 associated protein-1 (ubiquitin carboxy-terminal

hydrolase)

BCL2 B-cell CLL/lymphoma 2

BCL2A1 BCL2-related protein A1

BRAF B-Raf proto-oncogene, serine/threonine kinase

BUB1 BUB1 mitotic checkpoint serine/threonine kinase

CCND1 cyclin D1

CD63 CD63 molecule

CDH2 cadherin 2, type 1, N-cadherin (neuronal)

CDK4 cyclin-dependent kinase 4

CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1)

CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1)

CDKN2A cyclin-dependent kinase inhibitor 2A

CENPN centromere protein N

CSPG4 chondroitin sulfate proteoglycan 4

CTGF connective tissue growth factor

CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa

EGFR epidermal growth factor receptor

ERBB4 erb-b2 receptor tyrosine kinase 4

ETV1 ets variant 1

FGF2 fibroblast growth factor 2 (basic)

FGFR2 fibroblast growth factor receptor 2

FN1 fibronectin 1

FOXD1 forkhead box D1

GDF15 growth differentiation factor 15

GNA11 guanine nucleotide binding protein (G protein), alpha 11 (Gq class)

GNAQ guanine nucleotide binding protein (G protein), q polypeptide

GRIN2A glutamate receptor, ionotropic, N-methyl D-aspartate 2A

GRM3 glutamate receptor, metabotropic 3

HMGA2 high mobility group AT-hook 2

IL1B interleukin 1, beta

IL8 interleukin 8

KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

LGALS3 lectin, galactoside-binding, soluble, 3

MAGEA1 melanoma antigen family A1

MAGEA12 melanoma antigen family A12

MAGEA3 melanoma antigen family A3

MAP2K1 mitogen-activated protein kinase kinase 1

MAP2K2 mitogen-activated protein kinase kinase 2

MAP3K5 mitogen-activated protein kinase kinase kinase 5

MAP3K9 mitogen-activated protein kinase kinase kinase 9

MDM4 MDM4, p53 regulator

MET MET proto-oncogene, receptor tyrosine kinase

MITF microphthalmia-associated transcription factor

MKI67 marker of proliferation Ki-67

MLANA melan-A

MMP2 matrix metallopeptidase 2

MMP9 matrix metallopeptidase 9

MT1A metallothionein 1A

MT2A metallothionein 2A

MYC v-Myc Avian Myelocytomatosis Viral Oncogene Homolog

NCOA3 nuclear receptor coactivator 3

NEDD9 neural precursor cell expressed, developmentally down- regulated 9

NF1 neurofibromin 1

NRAS neuroblastoma RAS viral (v-ras) oncogene homolog

PAX3 paired box 3

PHACTR1 phosphatase and actin regulator 1

PIK3CA phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha

PMEL premelanosome protein

POSTN periostin, osteoblast specific factor

PPP6C protein phosphatase 6, catalytic subunit

PRAME preferentially associated melanoma antigen

PREX1 phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1

PREX2 phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2

PTEN phosphatase and tensin homolog

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RGS1 regulator of G-protein signaling 1

SERPINA3 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3

SERPINE1 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

SLC45A2 solute carrier family 45, member 2

SLC45A2 solute carrier family 45, member 2

SNX3 sorting nexin 3

SPP1 secreted phosphoprotein 1

SPRY4 sprouty homolog 4 (Drosophila)

STK19 serine/threonine kinase 19

TACC1 transforming Acidic Coiled-Coil Containing Protein 1

TERT telomerase reverse transcriptase

TP53 tumor protein p53

TRIM51 tripartite Motif-Containing 51

TRRAP transformation/transcription domain-associated protein

TUBB4A tubulin, beta 4A class IVa

TYR tyrosinase

UPP1 uridine phosphorylase 1

VEGFA vascular endothelial growth factor A

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a