Human Ferroptosis PCR Primer Library

Contains 88 primer sets directed against ferroptosis genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ACSF2 acyl-CoA synthetase family member 2

ACSL1 acyl-CoA synthetase long-chain family member 1

ACSL3 acyl-CoA synthetase long-chain family member 3

ACSL4 acyl-CoA synthetase long-chain family member 4

ACSL5 acyl-CoA synthetase long-chain family member 5

ACSL6 acyl-CoA synthetase long-chain family member 6

AKR1B1 aldo-keto reductase family 1, member B1 (aldose reductase)

AKR1B10 aldo-keto reductase family 1, member B10 (aldose reductase)

AKR1C1 aldo-keto reductase family 1, member C1

ALDH1A1 aldehyde dehydrogenase 1 family, member A1

ALOX15 arachidonate 15-lipoxygenase

ATG5 autophagy related 5

ATG7 autophagy related 7

ATP5G3 ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9)

BRAF B-Raf proto-oncogene, serine/threonine kinase

CARS1 cysteinyl-TRNA Synthetase 1 

CHAC1 ChaC glutathione-specific gamma-glutamylcyclotransferase 1

CISD1 CDGSH iron sulfur domain 1

CP ceruloplasmin (ferroxidase)

CS citrate synthase

CYBB cytochrome b-245, beta polypeptide

DMT1 dementia, familial nonspecific

EMC2 ER membrane protein complex subunit 2

EPRS glutamyl-prolyl-tRNA synthetase

FTH1 ferritin, heavy polypeptide 1

FTL ferritin, light polypeptide

FTMT ferritin mitochondrial

GCLC glutamate-cysteine ligase, catalytic subunit

GCLM glutamate-cysteine ligase, modifier subunit

GLS2 glutaminase 2 (liver, mitochondrial)

GOT1 glutamic-oxaloacetic transaminase 1, soluble

GPX4 glutathione peroxidase 4

GSS glutathione synthetase

GSTA1 glutathione S-transferase alpha 1

GSTP1 glutathione S-transferase pi 1

HAMP hepcidin antimicrobial peptide

HARS histidyl-tRNA synthetase

HEPH hephaestin

HFE homeostatic Iron Regulator

HMOX1 heme oxygenase 1

HMOX2 heme oxygenase 2

HRAS Harvey rat sarcoma viral oncogene homolog

HSF1 heat shock transcription factor 1

HSPB1 heat shock 27kDa protein 1

IREB2 iron-responsive element binding protein 2

KEAP1 kelch-like ECH-associated protein 1

KRAS Kirsten rat sarcoma viral oncogene homolog

LOX lysyl oxidase

LPCAT3 lysophosphatidylcholine acyltransferase 3

MAP1LC3A microtubule-associated protein 1 light chain 3 alpha

MAP1LC3B microtubule-associated protein 1 light chain 3 beta

MAP1LC3C microtubule-associated protein 1 light chain 3 gamma

NCOA4 nuclear receptor coactivator 4

NFE2L2 nuclear factor, erythroid 2-like 2

NOX1 NADPH Oxidase 1

NOX3 NADPH Oxidase 3

NOX4 NADPH Oxidase 4

NQO1 NAD(P)H dehydrogenase, quinone 1

NRAS neuroblastoma RAS viral (v-ras) oncogene homolog

NFE2L2 nuclear Factor, Erythroid 2 Like 2

PANX2 pannexin 2

PCBP1 poly(rC) binding protein 1

PCBP2 poly(rC) binding protein 2

PPARG peroxisome proliferator-activated receptor gamma

PRDX6 peroxiredoxin 6

PRNP prion protein

PTGES2 prostaglandin E synthase 2

BBC3 BCL2 Binding Component 3

RPL8 ribosomal protein L8

SAT1 spermidine/spermine N1-acetyltransferase 1

SAT2 spermidine/spermine N1-acetyltransferase family member 2

SLC11A2 solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2

SLC1A5 solute carrier family 1 (neutral amino acid transporter), member 5

SLC39A14 solute carrier family 39 (zinc transporter), member 14

SLC39A8 solute carrier family 39 (zinc transporter), member 8

SLC3A2 solute carrier family 3 (amino acid transporter heavy chain), member 2

SLC40A1 solute carrier family 40 (iron-regulated transporter), member 1

SLC7A11 solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11

SQSTM1 sequestosome 1

STEAP3 STEAP family member 3, metalloreductase

STIM1 stromal interaction molecule 1

TF transferrin

TFR2 Transferrin Receptor 2

TFRC transferrin receptor

TP53 tumor protein p53

TXNRD1 thioredoxin reductase 1

VDAC2 voltage-dependent anion channel 2

VDAC3 voltage-dependent anion channel 3

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a