Home > PCR Arrays > Ferroptosis

Ferroptosis

Ferroptosis is a novel, non-apoptotic type of cell death displaying unique features and is involved in various diseases including cancer. Ferroptosis is an oxidative, iron-dependent form of cell death and is distinct from apoptosis, classic necrosis, autophagy and other forms of cell death. It is triggered through inactivation of glutathione (GSH)-dependent antioxidant defenses, which results in the accumulation of toxic lipid reactive oxidative species (ROS). Depletion of plasma membrane polyunsaturated fatty acids also occurs. Ferroptosis has been implicated in the pathological death of brain tissues exposed to high levels of glutamate, and kidney and heart tissues subjected to ischemia–reperfusion injury. With respect to cancer, ferroptosis may act as an endogenous tumor suppressive mechanism downstream of p53. Recent studies have identified a number of genes required for ferroptosis, including those involved in lipid, iron and amino acid metabolism. The ferroptosis PCR primer library contains 88 genes directed at this important cell death pathway along with 8 housekeeping genes.

Human Ferroptosis PCR Primer Library
A ready-to-use Human Ferroptosis PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against ferroptosis genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ACSF2 acyl-CoA synthetase family member 2

ACSL1 acyl-CoA synthetase long-chain family member 1

ACSL3 acyl-CoA synthetase long-chain family member 3

ACSL4 acyl-CoA synthetase long-chain family member 4

ACSL5 acyl-CoA synthetase long-chain family member 5

ACSL6 acyl-CoA synthetase long-chain family member 6

AKR1B1 aldo-keto reductase family 1, member B1 (aldose reductase)

AKR1B10 aldo-keto reductase family 1, member B10 (aldose reductase)

AKR1C1 aldo-keto reductase family 1, member C1

ALDH1A1 aldehyde dehydrogenase 1 family, member A1

ALOX15 arachidonate 15-lipoxygenase

ATG5 autophagy related 5

ATG7 autophagy related 7

ATP5G3 ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9)

BRAF B-Raf proto-oncogene, serine/threonine kinase

CARS1 cysteinyl-TRNA Synthetase 1

CHAC1 ChaC glutathione-specific gamma-glutamylcyclotransferase 1

CISD1 CDGSH iron sulfur domain 1

CP ceruloplasmin (ferroxidase)

CS citrate synthase

CYBB cytochrome b-245, beta polypeptide

DMT1 dementia, familial nonspecific

EMC2 ER membrane protein complex subunit 2

EPRS glutamyl-prolyl-tRNA synthetase

FTH1 ferritin, heavy polypeptide 1

FTL ferritin, light polypeptide

FTMT ferritin mitochondrial

GCLC glutamate-cysteine ligase, catalytic subunit

GCLM glutamate-cysteine ligase, modifier subunit

GLS2 glutaminase 2 (liver, mitochondrial)

GOT1 glutamic-oxaloacetic transaminase 1, soluble

GPX4 glutathione peroxidase 4

GSS glutathione synthetase

GSTA1 glutathione S-transferase alpha 1

GSTP1 glutathione S-transferase pi 1

HAMP hepcidin antimicrobial peptide

HARS histidyl-tRNA synthetase

HEPH hephaestin

HFE homeostatic Iron Regulator

HMOX1 heme oxygenase 1

HMOX2 heme oxygenase 2

HRAS Harvey rat sarcoma viral oncogene homolog

HSF1 heat shock transcription factor 1

HSPB1 heat shock 27kDa protein 1

IREB2 iron-responsive element binding protein 2

KEAP1 kelch-like ECH-associated protein 1

KRAS Kirsten rat sarcoma viral oncogene homolog

LOX lysyl oxidase

LPCAT3 lysophosphatidylcholine acyltransferase 3

MAP1LC3A microtubule-associated protein 1 light chain 3 alpha

MAP1LC3B microtubule-associated protein 1 light chain 3 beta

MAP1LC3C microtubule-associated protein 1 light chain 3 gamma

NCOA4 nuclear receptor coactivator 4

NFE2L2 nuclear factor, erythroid 2-like 2

NOX1 NADPH Oxidase 1

NOX3 NADPH Oxidase 3

NOX4 NADPH Oxidase 4

NQO1 NAD(P)H dehydrogenase, quinone 1

NRAS neuroblastoma RAS viral (v-ras) oncogene homolog

NFE2L2 nuclear Factor, Erythroid 2 Like 2

PANX2 pannexin 2

PCBP1 poly(rC) binding protein 1

PCBP2 poly(rC) binding protein 2

PPARG peroxisome proliferator-activated receptor gamma

PRDX6 peroxiredoxin 6

PRNP prion protein

PTGES2 prostaglandin E synthase 2

BBC3 BCL2 Binding Component 3

RPL8 ribosomal protein L8

SAT1 spermidine/spermine N1-acetyltransferase 1

SAT2 spermidine/spermine N1-acetyltransferase family member 2

SLC11A2 solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2

SLC1A5 solute carrier family 1 (neutral amino acid transporter), member 5

SLC39A14 solute carrier family 39 (zinc transporter), member 14

SLC39A8 solute carrier family 39 (zinc transporter), member 8

SLC3A2 solute carrier family 3 (amino acid transporter heavy chain), member 2

SLC40A1 solute carrier family 40 (iron-regulated transporter), member 1

SLC7A11 solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11

SQSTM1 sequestosome 1

STEAP3 STEAP family member 3, metalloreductase

STIM1 stromal interaction molecule 1

TF transferrin

TFR2 Transferrin Receptor 2

TFRC transferrin receptor

TP53 tumor protein p53

TXNRD1 thioredoxin reductase 1

VDAC2 voltage-dependent anion channel 2

VDAC3 voltage-dependent anion channel 3

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
HFER-I
Mouse Ferroptosis PCR Primer Library
A ready-to-use Mouse Ferroptosis PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against ferroptosis genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

Acsf2 acyl-CoA synthetase family member 2

Acsl1 acyl-CoA synthetase long-chain family member 1

Acsl3 acyl-CoA synthetase long-chain family member 3

Acsl4 acyl-CoA synthetase long-chain family member 4

Acsl5 acyl-CoA synthetase long-chain family member 5

Acsl6 acyl-CoA synthetase long-chain family member 6

Akr1b3 aldo-keto reductase family 1, member B1 (aldose reductase)

Akr1b8 aldo-keto reductase family 1, member B10 (aldose reductase)

AKR1C1 aldo-keto reductase family 1, member C1

Aldh1a1 aldehyde dehydrogenase 1 family, member A1

Alox15 arachidonate 15-lipoxygenase

Atg5 autophagy related 5

Atg7 autophagy related 7

Atp5mc3 ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9)

Braf B-Raf proto-oncogene, serine/threonine kinase

Cars1 cysteinyl-TRNA Synthetase 1

Chac1 ChaC glutathione-specific gamma-glutamylcyclotransferase 1

Cisd1 CDGSH iron sulfur domain 1

Cp ceruloplasmin (ferroxidase)

Cs citrate synthase

Cybb cytochrome b-245, beta polypeptide

DMT1 dementia, familial nonspecific

Emc2 ER membrane protein complex subunit 2

Eprs1 glutamyl-prolyl-tRNA synthetase

Fth1 ferritin, heavy polypeptide 1

Ftl ferritin, light polypeptide

Ftmt ferritin mitochondrial

Gclc glutamate-cysteine ligase, catalytic subunit

Gclm glutamate-cysteine ligase, modifier subunit

Gls2 glutaminase 2 (liver, mitochondrial)

Got1 glutamic-oxaloacetic transaminase 1, soluble

Gpx4 glutathione peroxidase 4

Gss glutathione synthetase

Gsta1 glutathione S-transferase alpha 1

Gstp1 glutathione S-transferase pi 1

Hamp hepcidin antimicrobial peptide

Hars1 histidyl-tRNA synthetase

Heph hephaestin

Hfe homeostatic Iron Regulator

Hmox1 heme oxygenase 1

Hmox2 heme oxygenase 2

Hras Harvey rat sarcoma viral oncogene homolog

Hsf1 heat shock transcription factor 1

Hspb1 heat shock 27kDa protein 1

Ireb2 iron-responsive element binding protein 2

Keap1 kelch-like ECH-associated protein 1

Kras Kirsten rat sarcoma viral oncogene homolog

Lox lysyl oxidase

Lpcat3 lysophosphatidylcholine acyltransferase 3

Map1lc3a microtubule-associated protein 1 light chain 3 alpha

Map1lc3b microtubule-associated protein 1 light chain 3 beta

MAP1LC3C microtubule-associated protein 1 light chain 3 gamma

Ncoa4 nuclear receptor coactivator 4

Nfe2l2 nuclear factor, erythroid 2-like 2

Nox1 NADPH Oxidase 1

Nox3 NADPH Oxidase 3

Nox4 NADPH Oxidase 4

Nqo1 NAD(P)H dehydrogenase, quinone 1

Nras neuroblastoma RAS viral (v-ras) oncogene homolog

Nfe2l1 nuclear factor, erythroid 2-like 1

Panx2 pannexin 2

Pcbp1 poly(rC) binding protein 1

Pcbp2 poly(rC) binding protein 2

Pparg peroxisome proliferator-activated receptor gamma

Prdx6 peroxiredoxin 6

Prnp prion protein

Ptges2 prostaglandin E synthase 2

Bbc3 BCL2 Binding Component 3

Rpl8 ribosomal protein L8

Sat1 spermidine/spermine N1-acetyltransferase 1

Sat2 spermidine/spermine N1-acetyltransferase family member 2

Slc11a2 solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2

Slc1a5 solute carrier family 1 (neutral amino acid transporter), member 5

Slc39a14 solute carrier family 39 (zinc transporter), member 14

Slc39a8 solute carrier family 39 (zinc transporter), member 8

Slc3a2 solute carrier family 3 (amino acid transporter heavy chain), member 2

Slc40a1 solute carrier family 40 (iron-regulated transporter), member 1

Slc7a11 solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11

Sqstm1 sequestosome 1

Steap3 STEAP family member 3, metalloreductase

Stim1 stromal interaction molecule 1

Tf transferrin

Tfr2 Transferrin Receptor 2

Tfrc transferrin receptor

Trp53 tumor protein p53

Txnrd1 thioredoxin reductase 1

Vdac2 voltage-dependent anion channel 2

Vdac3 voltage-dependent anion channel 3

Actb Actin, beta

B2m Beta-2-microglobulin

Gapdh Glyceraldehyde-3-phosphate dehydrogenase

Gusb Glucuronidase, beta

Hprt Hypoxanthine phosphoribosyltransferase 1

Pgk1 Phosphoglycerate kinase 1

Ppia Peptidylprolyl isomerase A

Rpl13a Ribosomal protein L13a
$495.00
MFER-I