Human ER Stress (UPR) Primer Library

Contains 88 primer sets directed against ER stress genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ADM2 adrenomedullin 2

AMFR autocrine motility factor receptor, E3 ubiquitin protein ligase

ASNS asparagine synthetase (glutamine-hydrolyzing)

ATF4 activating transcription factor 4

ATF6 activating transcription factor 6

ATF6B activating transcription factor 6 beta

ATXN3 ataxin 3

BAX BCL2-associated X protein

BEX2 brain expressed X-linked 2

CALR calreticulin

CANX calnexin

CCT2 chaperonin containing TCP1, subunit 2

CCT4 chaperonin containing TCP1, subunit 4

CEBPB CCAAT/enhancer binding protein (C/EBP), beta

CREB1 cAMP responsive element binding protein 1

CREB3 cAMP responsive element binding protein 3

CREBBP CREB binding protein

DDIT3 DNA-damage-inducible transcript 3

DERL1 derlin 1

DNAJB9 DnaJ (Hsp40) homolog, subfamily B, member 9

DNAJC10 DnaJ (Hsp40) homolog, subfamily C, member 10

DNAJC3 DnaJ (Hsp40) homolog, subfamily C, member 3

EDEM1 ER degradation enhancer, mannosidase alpha-like 1

EIF2A In multiple Geneids

EIF2AK3 eukaryotic translation initiation factor 2-alpha kinase 3

ERN1 endoplasmic reticulum to nucleus signaling 1

ERN2 endoplasmic reticulum to nucleus signaling 2

ERO1L ERO1-like (S. cerevisiae)

ERO1LB ERO1-like beta (S. cerevisiae)

ERP44 endoplasmic reticulum protein 44

FBXO6 F-box protein 6

GANAB In multiple Geneids

GANC glucosidase, alpha; neutral C

GINS2 GINS complex subunit 2 (Psf2 homolog)

HERPUD1 homocysteine-inducible, endoplasmic reticulum stress- inducible, ubiquitin-like domain member 1

HSPA1A heat shock 70kDa protein 1A

HSPA1B heat shock 70kDa protein 1B

HSPA4 heat shock 70kDa protein 4

HSPA4L heat shock 70kDa protein 4-like

HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

HSPA6 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

HTRA2 HtrA serine peptidase 2

HTRA4 HtrA serine peptidase 4

INHBA Inhibin Subunit Beta A

INHBB Inhibin Subunit Beta B

INSIG2 insulin induced gene 2

KCNMB3 potassium channel subfamily M regulatory beta subunit 3

MANF mesencephalic astrocyte-derived neurotrophic factor

MAPK3 mitogen-activated protein kinase 3

MAPK8 mitogen-activated protein kinase 8

MAPK9 mitogen-activated protein kinase 9

MBTPS1 membrane-bound transcription factor peptidase, site 1

MBTPS2 membrane-bound transcription factor peptidase, site 2

MCM5 minichromosome maintenance complex component 5

NFKB1 nuclear Factor Kappa B Subunit 1

NPLOC4 nuclear protein localization 4 homolog (S. cerevisiae)

NUCB1 nucleobindin 1

OS9 osteosarcoma amplified 9, endoplasmic reticulum lectin

PCNA proliferating cell nuclear antigen

PDIA3 protein disulfide isomerase family A, member 3

PFDN5 prefoldin subunit 5

PPP1R15A protein phosphatase 1, regulatory subunit 15A

PRKCSH protein kinase C substrate 80K-H

RNF5 ring finger protein 5, E3 ubiquitin protein ligase

RPN1 ribophorin I

RRM1 ribonucleotide reductase M1

RRM2 ribonucleotide reductase M2

SCAP SREBF chaperone

SEC63 SEC63 homolog (S. cerevisiae)

SEL1L sel-1 suppressor of lin-12-like (C. elegans)

SERP1 stress associated endoplasmic reticulum protein 1

SIL1 SIL1 nucleotide exchange factor

SLC17A2 solute carrier family 17, member 2

SYVN1 synovial apoptosis inhibitor 1, synoviolin

TCP1 t-complex 1

TOR1A torsin family 1, member A (torsin A)

TRIB3 tribbles pseudokinase 3

TYMS thymidylate synthetase

UBE2G2 Ubiquitin Conjugating Enzyme E2 G2

UBXN4 UBX domain protein 4

UFD1L ubiquitin fusion degradation 1 like (yeast)

UGGT1 UDP-glucose glycoprotein glucosyltransferase 1

UHRF1 In multiple Geneids

USP14 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)

VCP valosin containing protein

VIMP VCP-interacting membrane selenoprotein

XBP1 X-box binding protein 1

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a