ER Stress (UPR) Pathway

Diseases, such as those of the neurvous system involve the accumulation of misfolded proteins in various tissues. The proteostasis network controls protein folding, trafficking and degradation. Disruption of these processes including disturbances in the function of the endoplasmic reticulum (ER) contribute to abnormal protein aggregation. The unfolded protein response (UPR) is a phenomenon that occurs following ER stress. There are a number of genes/proteins involved in this signaling pathway. The ER stress (UPR) primer library contains genes that participate in this pathway and may help researchers elucidate the function of ER stress in various neurological diseases.

A ready-to-use Human ER Stress (UPR) PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against ER stress genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ADM2 adrenomedullin 2

AMFR autocrine motility factor receptor, E3 ubiquitin protein ligase

ASNS asparagine synthetase (glutamine-hydrolyzing)

ATF4 activating transcription factor 4

ATF6 activating transcription factor 6

ATF6B activating transcription factor 6 beta

ATXN3 ataxin 3

BAX BCL2-associated X protein

BEX2 brain expressed X-linked 2

CALR calreticulin

CANX calnexin

CCT2 chaperonin containing TCP1, subunit 2

CCT4 chaperonin containing TCP1, subunit 4

CEBPB CCAAT/enhancer binding protein (C/EBP), beta

CREB1 cAMP responsive element binding protein 1

CREB3 cAMP responsive element binding protein 3

CREBBP CREB binding protein

DDIT3 DNA-damage-inducible transcript 3

DERL1 derlin 1

DNAJB9 DnaJ (Hsp40) homolog, subfamily B, member 9

DNAJC10 DnaJ (Hsp40) homolog, subfamily C, member 10

DNAJC3 DnaJ (Hsp40) homolog, subfamily C, member 3

EDEM1 ER degradation enhancer, mannosidase alpha-like 1

EIF2A In multiple Geneids

EIF2AK3 eukaryotic translation initiation factor 2-alpha kinase 3

ERN1 endoplasmic reticulum to nucleus signaling 1

ERN2 endoplasmic reticulum to nucleus signaling 2

ERO1L ERO1-like (S. cerevisiae)

ERO1LB ERO1-like beta (S. cerevisiae)

ERP44 endoplasmic reticulum protein 44

FBXO6 F-box protein 6

GANAB In multiple Geneids

GANC glucosidase, alpha; neutral C

GINS2 GINS complex subunit 2 (Psf2 homolog)

HERPUD1 homocysteine-inducible, endoplasmic reticulum stress- inducible, ubiquitin-like domain member 1

HSPA1A heat shock 70kDa protein 1A

HSPA1B heat shock 70kDa protein 1B

HSPA4 heat shock 70kDa protein 4

HSPA4L heat shock 70kDa protein 4-like

HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

HSPA6 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

HTRA2 HtrA serine peptidase 2

HTRA4 HtrA serine peptidase 4

INHBA Inhibin Subunit Beta A

INHBB Inhibin Subunit Beta B

INSIG2 insulin induced gene 2

KCNMB3 potassium channel subfamily M regulatory beta subunit 3

MANF mesencephalic astrocyte-derived neurotrophic factor

MAPK3 mitogen-activated protein kinase 3

MAPK8 mitogen-activated protein kinase 8

MAPK9 mitogen-activated protein kinase 9

MBTPS1 membrane-bound transcription factor peptidase, site 1

MBTPS2 membrane-bound transcription factor peptidase, site 2

MCM5 minichromosome maintenance complex component 5

NFKB1 nuclear Factor Kappa B Subunit 1

NPLOC4 nuclear protein localization 4 homolog (S. cerevisiae)

NUCB1 nucleobindin 1

OS9 osteosarcoma amplified 9, endoplasmic reticulum lectin

PCNA proliferating cell nuclear antigen

PDIA3 protein disulfide isomerase family A, member 3

PFDN5 prefoldin subunit 5

PPP1R15A protein phosphatase 1, regulatory subunit 15A

PRKCSH protein kinase C substrate 80K-H

RNF5 ring finger protein 5, E3 ubiquitin protein ligase

RPN1 ribophorin I

RRM1 ribonucleotide reductase M1

RRM2 ribonucleotide reductase M2

SCAP SREBF chaperone

SEC63 SEC63 homolog (S. cerevisiae)

SEL1L sel-1 suppressor of lin-12-like (C. elegans)

SERP1 stress associated endoplasmic reticulum protein 1

SIL1 SIL1 nucleotide exchange factor

SLC17A2 solute carrier family 17, member 2

SYVN1 synovial apoptosis inhibitor 1, synoviolin

TCP1 t-complex 1

TOR1A torsin family 1, member A (torsin A)

TRIB3 tribbles pseudokinase 3

TYMS thymidylate synthetase

UBE2G2 Ubiquitin Conjugating Enzyme E2 G2

UBXN4 UBX domain protein 4

UFD1L ubiquitin fusion degradation 1 like (yeast)

UGGT1 UDP-glucose glycoprotein glucosyltransferase 1

UHRF1 In multiple Geneids

USP14 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)

VCP valosin containing protein

VIMP VCP-interacting membrane selenoprotein

XBP1 X-box binding protein 1

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
HEPR-I
A ready-to-use Mouse ER Stress (UPR) PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against ER stress genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ADM2 adrenomedullin 2

AMFR autocrine motility factor receptor, E3 ubiquitin protein ligase

ASNS asparagine synthetase (glutamine-hydrolyzing)

ATF4 activating transcription factor 4

ATF6 activating transcription factor 6

ATF6B activating transcription factor 6 beta

ATXN3 ataxin 3

BAX BCL2-associated X protein

BEX2 brain expressed X-linked 2

CALR calreticulin

CANX calnexin

CCT2 chaperonin containing TCP1, subunit 2

CCT4 chaperonin containing TCP1, subunit 4

CEBPB CCAAT/enhancer binding protein (C/EBP), beta

CREB1 cAMP responsive element binding protein 1

CREB3 cAMP responsive element binding protein 3

CREBBP CREB binding protein

DDIT3 DNA-damage-inducible transcript 3

DERL1 derlin 1

DNAJB9 DnaJ (Hsp40) homolog, subfamily B, member 9

DNAJC10 DnaJ (Hsp40) homolog, subfamily C, member 10

DNAJC3 DnaJ (Hsp40) homolog, subfamily C, member 3

EDEM1 ER degradation enhancer, mannosidase alpha-like 1

EIF2A In multiple Geneids

EIF2AK3 eukaryotic translation initiation factor 2-alpha kinase 3

ERN1 endoplasmic reticulum to nucleus signaling 1

ERN2 endoplasmic reticulum to nucleus signaling 2

ERO1L ERO1-like (S. cerevisiae)

ERO1LB ERO1-like beta (S. cerevisiae)

ERP44 endoplasmic reticulum protein 44

FBXO6 F-box protein 6

GANAB In multiple Geneids

GANC glucosidase, alpha; neutral C

GINS2 GINS complex subunit 2 (Psf2 homolog)

HERPUD1 homocysteine-inducible, endoplasmic reticulum stress- inducible, ubiquitin-like domain member 1

HSPA1A heat shock 70kDa protein 1A

HSPA1B heat shock 70kDa protein 1B

HSPA4 heat shock 70kDa protein 4

HSPA4L heat shock 70kDa protein 4-like

HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

HSPA6 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

HTRA2 HtrA serine peptidase 2

HTRA4 HtrA serine peptidase 4

INHBA Inhibin Subunit Beta A

INHBB Inhibin Subunit Beta B

INSIG2 insulin induced gene 2

KCNMB3 potassium channel subfamily M regulatory beta subunit 3

MANF mesencephalic astrocyte-derived neurotrophic factor

MAPK3 mitogen-activated protein kinase 3

MAPK8 mitogen-activated protein kinase 8

MAPK9 mitogen-activated protein kinase 9

MBTPS1 membrane-bound transcription factor peptidase, site 1

MBTPS2 membrane-bound transcription factor peptidase, site 2

MCM5 minichromosome maintenance complex component 5

NFKB1 nuclear Factor Kappa B Subunit 1

NPLOC4 nuclear protein localization 4 homolog (S. cerevisiae)

NUCB1 nucleobindin 1

OS9 osteosarcoma amplified 9, endoplasmic reticulum lectin

PCNA proliferating cell nuclear antigen

PDIA3 protein disulfide isomerase family A, member 3

PFDN5 prefoldin subunit 5

PPP1R15A protein phosphatase 1, regulatory subunit 15A

PRKCSH protein kinase C substrate 80K-H

RNF5 ring finger protein 5, E3 ubiquitin protein ligase

RPN1 ribophorin I

RRM1 ribonucleotide reductase M1

RRM2 ribonucleotide reductase M2

SCAP SREBF chaperone

SEC63 SEC63 homolog (S. cerevisiae)

SEL1L sel-1 suppressor of lin-12-like (C. elegans)

SERP1 stress associated endoplasmic reticulum protein 1

SIL1 SIL1 nucleotide exchange factor

SLC17A2 solute carrier family 17, member 2

SYVN1 synovial apoptosis inhibitor 1, synoviolin

TCP1 t-complex 1

TOR1A torsin family 1, member A (torsin A)

TRIB3 tribbles pseudokinase 3

TYMS thymidylate synthetase

UBE2G2 Ubiquitin Conjugating Enzyme E2 G2

UBXN4 UBX domain protein 4

UFD1L ubiquitin fusion degradation 1 like (yeast)

UGGT1 UDP-glucose glycoprotein glucosyltransferase 1

UHRF1 In multiple Geneids

USP14 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)

VCP valosin containing protein

VIMP VCP-interacting membrane selenoprotein

XBP1 X-box binding protein 1

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
MEPR-I