Human DNA Damage Signaling Primer Library I

Contains 88 primer sets directed against DNA damage signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix



GENE LIST:

Abl1 C-abl oncogene 1, non-receptor tyrosine kinase

Apex1 Apurinic/apyrimidinic endonuclease 1

Atm Ataxia telangiectasia mutated homolog (human)

Atr Ataxia telangiectasia and Rad3 related

Atrx Alpha thalassemia/mental retardation syndrome X-linked homolog (human)

Bax BCL2-associated X protein

Blm Bloom syndrome, RecQ helicase-like

Brca1 Breast cancer 1

Brca2 Breast cancer 2

Brip1 BRCA1 interacting protein C-terminal helicase 1

Cdc25a Cell division cycle 25 homolog A

Cdc25b Cell division cycle 25 homolog B

Cdc25c Cell division cycle 25 homolog C

Cdkn1a Cyclin-dependent kinase inhibitor 1A (P21)

Chek1 Checkpoint kinase 1 homolog

Chek2 CHK2 checkpoint homolog

Dclre1a DNA cross-link repair 1A, PSO2 homolog

Ddb2 Damage specific DNA binding protein 2

Ddit3 DNA-damage inducible transcript 3

Ercc1 Excision repair cross-complementing rodent repair deficiency, complementation group 1

Ercc2 Excision repair cross-complementing rodent repair deficiency, complementation group 2

Exo1 Exonuclease 1

Fanca Fanconi anemia, complementation group A

Fancc Fanconi anemia, complementation group C

Fancd2 Fanconi anemia, complementation group D2

Fancg Fanconi anemia, complementation group G

Fen1 Flap structure specific endonuclease 1

Gadd45a Growth arrest and DNA-damage-inducible 45 alpha

Gadd45b Growth arrest and DNA-damage-inducible 45 beta

Gadd45g Growth arrest and DNA-damage-inducible 45 gamma

H2afx H2A histone family, member X

Hus1 Hus1 homolog

Lig1 Ligase I, DNA, ATP-dependent

Mbd4 Methyl-CpG binding domain protein 4

Mcph1 Microcephaly, primary autosomal recessive 1

Mdc1 Mediator of DNA damage checkpoint 1

Mgmt O-6-methylguanine-DNA methyltransferase

Mif Macrophage migration inhibitory factor

Mlh1 MutL homolog 1 (E. coli)

Mlh3 MutL homolog 3 (E coli)

Mpg N-methylpurine-DNA glycosylase

Mre11a Meiotic recombination 11 homolog A

Msh2 MutS homolog 2 (E. coli)

Msh3 MutS homolog 3 (E. coli)

Nbn Nibrin

Nthl1 Nth (endonuclease III)-like 1 (E.coli)

Ogg1 8-oxoguanine DNA-glycosylase 1

Parp1 Poly (ADP-ribose) polymerase family, member 1

Parp2 Poly (ADP-ribose) polymerase family, member 2

Pcna Proliferating cell nuclear antigen

Pms1 Postmeiotic segregation increased 1

Pms2 Postmeiotic segregation increased 2

Pole Polymerase (DNA directed), epsilon

Polh Polymerase (DNA directed), eta (RAD 30 related)

Poli Polymerase (DNA directed), iota

Ppm1d Protein phosphatase 1D magnesium-dependent, delta isoform

Ppp1r15a Protein phosphatase 1, regulatory subunit 15A

Prkdc Protein kinase, DNA activated, catalytic polypeptide

Pttg1 Pituitary tumor-transforming gene 1

Rad1 RAD1 homolog

Rad17 RAD17 homolog

Rad18 RAD18 homolog

Rad21 RAD21 homolog

Rad50 RAD50 homolog

Rad51 RAD51 homolog

Rad51b RAD51 homolog b

Rad51c RAD51 homolog c

Rad52 RAD52 homolog

Rad9a RAD9a homolog

Rev1 REV1 homolog

Rnf8 Ring finger protein 8

Rpa1 Replication protein A1

Smc1a Structural maintenance of chromosomes 1A

Smc3 Structural maintenace of chromosomes 3

Sumo1 SMT3 suppressor of mif two 3 homolog 1

Sumo2 SMT3 suppressor of mif two 3 homolog 2

Terf1 Telomeric repeat binding factor 1

Topbp1 Topoisomerase (DNA) II binding protein 1

Trp53 Transformation related protein 53

Trp53bp1 Transformation related protein 53 binding protein 1

Ung Uracil DNA glycosylase

Wrn Werner syndrome homolog

Xpa Xeroderma pigmentosum, complementation group A

Xpc Xeroderma pigmentosum, complementation group C

Xrcc1 X-ray repair complementing defective repair in Chinese hamster cells 1

Xrcc2 X-ray repair complementing defective repair in Chinese hamster cells 2

Xrcc3 X-ray repair complementing defective repair in Chinese hamster cells 3

Xrcc6 X-ray repair complementing defective repair in Chinese hamster cells 6

Actb Actin, beta

B2m Beta-2 microglobulin

Gapd glyceraldehyde-3-phosphate dehydrogenase

Gusb Glucuronidase, beta

Hprt1 Hypoxanthine guanine phosphoribosyl transferase 1

Pgk1 Phosphoglycerate kinase 1

Ppia Peptidylprolyl isomerase A

Rpl13a Ribosomal protein L13a