Cells are constantly exposed to DNA damage from replication errors, reactive oxygen species, radiation and chemicals. The DNA damage response (DDR) detects lesions through sensor kinases ATM, ATR and DNA-PK, which phosphorylate H2AX and checkpoint kinases CHEK1 and CHEK2. These signals activate p53 and other effectors that arrest the cell cycle, recruit DNA repair machinery, or trigger apoptosis or senescence if the damage cannot be repaired. Defects in DNA damage signaling cause genomic instability and are a hallmark of cancer.
A ready-to-use Mouse DNA Damage Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against DNA damage signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: Abl1 C-abl oncogene 1, non-receptor tyrosine kinase Apex1 Apurinic/apyrimidinic endonuclease 1 Atm Ataxia telangiectasia mutated homolog (human) Atr Ataxia telangiectasia and Rad3 related Atrx Alpha thalassemia/mental retardation syndrome X-linked homolog (human) Bax BCL2-associated X protein Blm Bloom syndrome, RecQ helicase-like Brca1 Breast cancer 1 Brca2 Breast cancer 2 Brip1 BRCA1 interacting protein C-terminal helicase 1 Cdc25a Cell division cycle 25 homolog A Cdc25b Cell division cycle 25 homolog B Cdc25c Cell division cycle 25 homolog C Cdkn1a Cyclin-dependent kinase inhibitor 1A (P21) Chek1 Checkpoint kinase 1 homolog Chek2 CHK2 checkpoint homolog Dclre1a DNA cross-link repair 1A, PSO2 homolog Ddb2 Damage specific DNA binding protein 2 Ddit3 DNA-damage inducible transcript 3 Ercc1 Excision repair cross-complementing rodent repair deficiency, complementation group 1 Ercc2 Excision repair cross-complementing rodent repair deficiency, complementation group 2 Exo1 Exonuclease 1 Fanca Fanconi anemia, complementation group A Fancc Fanconi anemia, complementation group C Fancd2 Fanconi anemia, complementation group D2 Fancg Fanconi anemia, complementation group G Fen1 Flap structure specific endonuclease 1 Gadd45a Growth arrest and DNA-damage-inducible 45 alpha Gadd45b Growth arrest and DNA-damage-inducible 45 beta Gadd45g Growth arrest and DNA-damage-inducible 45 gamma H2afx H2A histone family, member X Hus1 Hus1 homolog Lig1 Ligase I, DNA, ATP-dependent Mbd4 Methyl-CpG binding domain protein 4 Mcph1 Microcephaly, primary autosomal recessive 1 Mdc1 Mediator of DNA damage checkpoint 1 Mgmt O-6-methylguanine-DNA methyltransferase Mif Macrophage migration inhibitory factor Mlh1 MutL homolog 1 (E. coli) Mlh3 MutL homolog 3 (E coli) Mpg N-methylpurine-DNA glycosylase Mre11a Meiotic recombination 11 homolog A Msh2 MutS homolog 2 (E. coli) Msh3 MutS homolog 3 (E. coli) Nbn Nibrin Nthl1 Nth (endonuclease III)-like 1 (E.coli) Ogg1 8-oxoguanine DNA-glycosylase 1 Parp1 Poly (ADP-ribose) polymerase family, member 1 Parp2 Poly (ADP-ribose) polymerase family, member 2 Pcna Proliferating cell nuclear antigen Pms1 Postmeiotic segregation increased 1 Pms2 Postmeiotic segregation increased 2 Pole Polymerase (DNA directed), epsilon Polh Polymerase (DNA directed), eta (RAD 30 related) Poli Polymerase (DNA directed), iota Ppm1d Protein phosphatase 1D magnesium-dependent, delta isoform Ppp1r15a Protein phosphatase 1, regulatory subunit 15A Prkdc Protein kinase, DNA activated, catalytic polypeptide Pttg1 Pituitary tumor-transforming gene 1 Rad1 RAD1 homolog Rad17 RAD17 homolog Rad18 RAD18 homolog Rad21 RAD21 homolog Rad50 RAD50 homolog Rad51 RAD51 homolog Rad51b RAD51 homolog b Rad51c RAD51 homolog c Rad52 RAD52 homolog Rad9a RAD9a homolog Rev1 REV1 homolog Rnf8 Ring finger protein 8 Rpa1 Replication protein A1 Smc1a Structural maintenance of chromosomes 1A Smc3 Structural maintenace of chromosomes 3 Sumo1 SMT3 suppressor of mif two 3 homolog 1 Sumo2 SMT3 suppressor of mif two 3 homolog 2 Terf1 Telomeric repeat binding factor 1 Topbp1 Topoisomerase (DNA) II binding protein 1 Trp53 Transformation related protein 53 Trp53bp1 Transformation related protein 53 binding protein 1 Ung Uracil DNA glycosylase Wrn Werner syndrome homolog Xpa Xeroderma pigmentosum, complementation group A Xpc Xeroderma pigmentosum, complementation group C Xrcc1 X-ray repair complementing defective repair in Chinese hamster cells 1 Xrcc2 X-ray repair complementing defective repair in Chinese hamster cells 2 Xrcc3 X-ray repair complementing defective repair in Chinese hamster cells 3 Xrcc6 X-ray repair complementing defective repair in Chinese hamster cells 6 Actb Actin, beta B2m Beta-2 microglobulin Gapd glyceraldehyde-3-phosphate dehydrogenase Gusb Glucuronidase, beta Hprt1 Hypoxanthine guanine phosphoribosyl transferase 1 Pgk1 Phosphoglycerate kinase 1 Ppia Peptidylprolyl isomerase A Rpl13a Ribosomal protein L13a $495.00 |
A ready-to-use Human DNA Damage Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against DNA damage signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: Abl1 C-abl oncogene 1, non-receptor tyrosine kinase Apex1 Apurinic/apyrimidinic endonuclease 1 Atm Ataxia telangiectasia mutated homolog (human) Atr Ataxia telangiectasia and Rad3 related Atrx Alpha thalassemia/mental retardation syndrome X-linked homolog (human) Bax BCL2-associated X protein Blm Bloom syndrome, RecQ helicase-like Brca1 Breast cancer 1 Brca2 Breast cancer 2 Brip1 BRCA1 interacting protein C-terminal helicase 1 Cdc25a Cell division cycle 25 homolog A Cdc25b Cell division cycle 25 homolog B Cdc25c Cell division cycle 25 homolog C Cdkn1a Cyclin-dependent kinase inhibitor 1A (P21) Chek1 Checkpoint kinase 1 homolog Chek2 CHK2 checkpoint homolog Dclre1a DNA cross-link repair 1A, PSO2 homolog Ddb2 Damage specific DNA binding protein 2 Ddit3 DNA-damage inducible transcript 3 Ercc1 Excision repair cross-complementing rodent repair deficiency, complementation group 1 Ercc2 Excision repair cross-complementing rodent repair deficiency, complementation group 2 Exo1 Exonuclease 1 Fanca Fanconi anemia, complementation group A Fancc Fanconi anemia, complementation group C Fancd2 Fanconi anemia, complementation group D2 Fancg Fanconi anemia, complementation group G Fen1 Flap structure specific endonuclease 1 Gadd45a Growth arrest and DNA-damage-inducible 45 alpha Gadd45b Growth arrest and DNA-damage-inducible 45 beta Gadd45g Growth arrest and DNA-damage-inducible 45 gamma H2afx H2A histone family, member X Hus1 Hus1 homolog Lig1 Ligase I, DNA, ATP-dependent Mbd4 Methyl-CpG binding domain protein 4 Mcph1 Microcephaly, primary autosomal recessive 1 Mdc1 Mediator of DNA damage checkpoint 1 Mgmt O-6-methylguanine-DNA methyltransferase Mif Macrophage migration inhibitory factor Mlh1 MutL homolog 1 (E. coli) Mlh3 MutL homolog 3 (E coli) Mpg N-methylpurine-DNA glycosylase Mre11a Meiotic recombination 11 homolog A Msh2 MutS homolog 2 (E. coli) Msh3 MutS homolog 3 (E. coli) Nbn Nibrin Nthl1 Nth (endonuclease III)-like 1 (E.coli) Ogg1 8-oxoguanine DNA-glycosylase 1 Parp1 Poly (ADP-ribose) polymerase family, member 1 Parp2 Poly (ADP-ribose) polymerase family, member 2 Pcna Proliferating cell nuclear antigen Pms1 Postmeiotic segregation increased 1 Pms2 Postmeiotic segregation increased 2 Pole Polymerase (DNA directed), epsilon Polh Polymerase (DNA directed), eta (RAD 30 related) Poli Polymerase (DNA directed), iota Ppm1d Protein phosphatase 1D magnesium-dependent, delta isoform Ppp1r15a Protein phosphatase 1, regulatory subunit 15A Prkdc Protein kinase, DNA activated, catalytic polypeptide Pttg1 Pituitary tumor-transforming gene 1 Rad1 RAD1 homolog Rad17 RAD17 homolog Rad18 RAD18 homolog Rad21 RAD21 homolog Rad50 RAD50 homolog Rad51 RAD51 homolog Rad51b RAD51 homolog b Rad51c RAD51 homolog c Rad52 RAD52 homolog Rad9a RAD9a homolog Rev1 REV1 homolog Rnf8 Ring finger protein 8 Rpa1 Replication protein A1 Smc1a Structural maintenance of chromosomes 1A Smc3 Structural maintenace of chromosomes 3 Sumo1 SMT3 suppressor of mif two 3 homolog 1 Sumo2 SMT3 suppressor of mif two 3 homolog 2 Terf1 Telomeric repeat binding factor 1 Topbp1 Topoisomerase (DNA) II binding protein 1 Trp53 Transformation related protein 53 Trp53bp1 Transformation related protein 53 binding protein 1 Ung Uracil DNA glycosylase Wrn Werner syndrome homolog Xpa Xeroderma pigmentosum, complementation group A Xpc Xeroderma pigmentosum, complementation group C Xrcc1 X-ray repair complementing defective repair in Chinese hamster cells 1 Xrcc2 X-ray repair complementing defective repair in Chinese hamster cells 2 Xrcc3 X-ray repair complementing defective repair in Chinese hamster cells 3 Xrcc6 X-ray repair complementing defective repair in Chinese hamster cells 6 Actb Actin, beta B2m Beta-2 microglobulin Gapd glyceraldehyde-3-phosphate dehydrogenase Gusb Glucuronidase, beta Hprt1 Hypoxanthine guanine phosphoribosyl transferase 1 Pgk1 Phosphoglycerate kinase 1 Ppia Peptidylprolyl isomerase A Rpl13a Ribosomal protein L13a $495.00 |