Apoptosis is a tightly regulated form of programmed cell death that removes damaged, infected or unneeded cells during development and tissue homeostasis. It is triggered through the extrinsic pathway, in which death ligands such as FASL, TNF and TRAIL bind their receptors and activate caspase 8, or through the intrinsic (mitochondrial) pathway, in which BCL2 family proteins control release of cytochrome c and activation of APAF1 and caspase 9. Both pathways converge on the executioner caspases 3, 6 and 7, which dismantle the cell. Inhibitor of apoptosis (IAP) proteins and anti-apoptotic BCL2 family members restrain this process, and defects in apoptosis contribute to cancer, autoimmunity and neurodegeneration.
A ready-to-use Human Apoptosis AI PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against apoptosis genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: AIFM1 apoptosis-inducing factor, mitochondrion-associated, 1 APAF1 apoptotic peptidase activating factor 1 BAD BCL2-associated agonist of cell death BAK1 BCL2-antagonist/killer 1 BAX BCL2-associated X protein BBC3 BCL2 binding component 3 BCL2 B-cell CLL/lymphoma 2 BCL2L1 BCL2-like 1 BCL2L10 BCL2-like 10 (apoptosis facilitator) BCL2L2 BCL2-like 2 BID BH3 interacting domain death agonist BIRC2 baculoviral IAP repeat containing 2 BIRC3 baculoviral IAP repeat containing 3 BIRC4 baculoviral IAP repeat containing 4 BIRC5 baculoviral IAP repeat containing 5 BNIP3 BCL2/adenovirus E1B 19kDa interacting protein 3 BNIP3L BCL2/adenovirus E1B 19kDa interacting protein 3-like BOK BCL2-related ovarian killer CARD16 caspase recruitment domain family, member 16 CASP1 caspase 1, apoptosis-related cysteine peptidase CASP10 caspase 10, apoptosis-related cysteine peptidase CASP2 caspase 2, apoptosis-related cysteine peptidase CASP3 caspase 3, apoptosis-related cysteine peptidase CASP4 caspase 4, apoptosis-related cysteine peptidase CASP5 caspase 5, apoptosis-related cysteine peptidase CASP6 caspase 6, apoptosis-related cysteine peptidase CASP7 caspase 7, apoptosis-related cysteine peptidase CASP8 caspase 8, apoptosis-related cysteine peptidase CASP9 caspase 9, apoptosis-related cysteine peptidase CCAR1 cell division cycle and apoptosis regulator 1 CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) CFLAR CASP8 and FADD-like apoptosis regulator CHUK conserved helix-loop-helix ubiquitous kinase DAP death-associated protein DAXX death-domain associated protein DFFA DNA fragmentation factor, 45kDa, alpha polypeptide DFFB DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) DIABLO diablo, IAP-binding mitochondrial protein ENDOG endonuclease G FADD Fas (TNFRSF6)-associated via death domain FAIM Fas apoptotic inhibitory molecule FAS Fas cell surface death receptor FASLG Fas ligand (TNF superfamily, member 6) GADD45A growth arrest and DNA-damage-inducible, alpha GADD45B growth arrest and DNA-damage-inducible, beta GADD45G growth arrest and DNA-damage-inducible, gamma GAS2 growth arrest-specific 2 HMGB1 high mobility group box 1 HRK harakiri, BCL2 interacting protein IER3 immediate early response 3 ING1 inhibitor of growth family, member 1 ING4 inhibitor of growth family, member 4 INPP5K inositol polyphosphate-5-phosphatase K IRF1 interferon regulatory factor 1 JUND jun D proto-oncogene MADD MAP-kinase activating death domain MAP3K5 mitogen-activated protein kinase kinase kinase 5 MCL1 myeloid cell leukemia 1 NOL3 nucleolar protein 3 (apoptosis repressor with CARD domain) PIDD1 P53-Induced Death Domain Protein 1 PMAIP1 phorbol-12-myristate-13-acetate-induced protein 1 PMAIP1 phorbol-12-myristate-13-acetate-induced protein 1 PMAIP1 phorbol-12-myristate-13-acetate-induced protein 1 PPP1R15A protein phosphatase 1, regulatory subunit 15A PSEN1 presenilin 1 PSEN2 presenilin 2 RNF31 ring finger protein 31 SIVA1 SIVA1, apoptosis-inducing factor SLC25A4 solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4 TNF tumor necrosis factor TNFAIP3 tumor necrosis factor, alpha-induced protein 3 TNFRSF10A tumor necrosis factor receptor superfamily, member 10a TNFRSF10B tumor necrosis factor receptor superfamily, member 10b TNFRSF10C tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain TNFRSF10D tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain TNFSF10 tumor necrosis factor (ligand) superfamily, member 10 TP53 tumor protein p53 TP63 tumor protein p63 TP73 tumor protein p73 TRADD TNFRSF1A-associated via death domain TRAF1 TNF receptor-associated factor 1 TRAF2 TNF receptor-associated factor 2 TRAF3 TNF receptor-associated factor 3 TRAF4 TNF receptor-associated factor 4 TRAF5 TNF receptor-associated factor 5 TRAF6 TNF receptor-associated factor 6, E3 ubiquitin protein ligase TXNIP thioredoxin interacting protein ZBTB16 Zinc Finger And BTB Domain Containing 16 ACTB Actin, beta B2M Beta-2-microglobulin GAPD Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |