Home > PCR Arrays > Apoptosis

Apoptosis

Apoptosis or programmed cell death is a controlled and highly regulated process in mammalian cells. There are two main pathways: extrinsic an intrinsic. The extrinsic (death receptor) pathway relies on extracellular signals (ie Fas ligand, TRAIL) to activate proximal caspases (caspases 8 and 10) leading to cleavage and activation of the executioner caspases 3, 6 and 7. The intrinsic (mitochondrial) pathway requires disruption of the mitochondrial membrane causing release of the proteins Smac/Diablo, Omi/HtRrA2 and cytochrome C. Apoptosome formation subsequently occurs which results in caspase 9 activation followed by activation of the executioner caspases 3, 6 and 7. Sufficient overlap of these two major pathways occurs allowing extrinsic pathway agonists to influence intrinsic pathway activators and vice-versa. A number of upstream and downstream genes affect apoptosis and these genes are represented in this PCR array product.

Human Apoptosis Primer Library
A ready-to-use Human Apoptosis PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against apoptosis genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:



APAF1 Apoptotic protease activating factor

ATM Ataxia telangiectasia mutated

BAG1 BCL2-associated athanogene

BAG3 BCL2-associated athanogene 3

BAG4 BCL2-associated athanogene 4

BAK1 BCL2-antagonist/killer 1

BAX BCL2-associated X protein

BCL10 B-cell CLL/lymphoma 10

BCL2 B-cell CLL/lymphoma 2

BCL2A1 BCL2-related protein A1

BCL2L1 BCL2-like 1

BCL2L11 BCL2-like 11 (apoptosis facilitator)

BCL2L2 BCL2-like 2

BFAR Bifunctional apoptosis regulator

BIK BCL2-interacting killer (apoptosis-inducing)

BIRC1 Baculoviral IAP repeat-containing 1

BIRC2 Baculoviral IAP repeat-containing 2

BIRC3 Baculoviral IAP repeat-containing 3

BIRC4 Baculoviral IAP repeat-containing 4

BIRC5 Baculoviral IAP repeat-containing 5 (survivin)

BIRC6 Baculoviral IAP repeat-containing 6 (apollon)

BNIP3 BCL2/adenovirus E1B 19kDa interacting protein 3

BRE Brain and reproductive organ-expressed

BOK BCL2-related ovarian killer

CASP1 Caspase 1, apoptosis-related cysteine protease

CASP10 Caspase 10, apoptosis-related cysteine protease

CASP4 Caspase 4, apoptosis-related cysteine peptidase

CASP2 Caspase 2, apoptosis-related cysteine protease

CASP3 Caspase 3, apoptosis-related cysteine protease

CASP5 Caspase 5, apoptosis-related cysteine protease

CASP6 Caspase 6, apoptosis-related cysteine protease

CASP7 Caspase 7, apoptosis-related cysteine protease

CASP8 Caspase 8, apoptosis-related cysteine protease

CASP8AP2 CASP8 associated protein 2

CFLAR CASP8 and FADD-like apoptosis regulator

CHEK1 CHK1 checkpoint homolog (S. pombe)

CHEK2 CHK2 checkpoint homolog (S. pombe)

CIDEA Cell death-inducing DFFA-like effector a

CIDEB Cell death-inducing DFFA-like effector b

CRADD CASP2 and RIPK1 domain containing adaptor

DAPK1 Death-associated protein kinase 1

DAPK2 Death-associated protein kinase 2

DFFA DNA fragmentation factor, 45kDa, alpha polypeptide

DFFB DNA fragmentation factor, 40kDa, beta polypeptide

FADD Fas (TNFRSF6)-associated via death domain

GADD45A Growth arrest and DNA-damage-inducible, alpha

HRK Harakiri, BCL2 interacting protein

LTBR Lymphotoxin beta receptor (TNFR superfamily, member 3)

MCL1 Myeloid cell leukemia sequence 1 (BCL2-related)

MYD88 Myeloid differentiation primary response gene (88)

CARD4 Caspase recruitment domain family, member 4

FAS Fas (TNF receptor superfamily, member 6)

RIPK2 Receptor-interacting serine-threonine kinase 2

RPA3 Replication protein A3, 14kDa

TANK TRAF family member-associated NFKB activator

TNF Tumor necrosis factor (TNF superfamily, member 2)

TNFRSF10A Tumor necrosis factor receptor superfamily, member 10a

TNFRSF10B Tumor necrosis factor receptor superfamily, member 10b

TNFRSF10C Tumor necrosis factor receptor superfamily, member 10c

TNFRSF10D Tumor necrosis factor receptor superfamily, member 10d

TNFRSF11B Tumor necrosis factor receptor superfamily, member 11b

TNFRSF17 Tumor necrosis factor receptor superfamily, member 17

TNFRSF1A Tumor necrosis factor receptor superfamily, member 1A

TNFRSF21 Tumor necrosis factor receptor superfamily, member 21

CD40 CD40 antigen (TNF receptor superfamily member 5)

TNFRSF8 Tumor necrosis factor receptor superfamily, member 8

TNFRSF9 Tumor necrosis factor receptor superfamily, member 9

TNFSF10 Tumor necrosis factor (ligand) superfamily, member 10

TNFSF11 Tumor necrosis factor (ligand) superfamily, member 11

TNFSF13 Tumor necrosis factor (ligand) superfamily, member 12

TNFSF13B Tumor necrosis factor (ligand) superfamily, member 13b

TNFSF15 Tumor necrosis factor (ligand) superfamily, member 15

TNFSF18 Tumor necrosis factor (ligand) superfamily, member 18

TNFSF4 Tumor necrosis factor (ligand) superfamily, member 4

CD40LG CD40 ligand (TNF superfamily, member 5)

FASLG Fas ligand (TNF superfamily, member 6)

TNFSF7 Tumor necrosis factor (ligand) superfamily, member 7

TNFSF8 Tumor necrosis factor (ligand) superfamily, member 8

TNFSF9 Tumor necrosis factor (ligand) superfamily, member 9

TP53 Tumor protein p53 (Li-Fraumeni syndrome)

TP73L Tumor protein p73-like

TRAF1 TNF receptor-associated factor 1

TRAF2 TNF receptor-associated factor 2

TRAF3 TNF receptor-associated factor 3

TRAF4 TNF receptor-associated factor 4

TRAF5 TNF receptor-associated factor 5

TRAF6 TNF receptor-associated factor 6

TRIP TRAF interacting protein

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a

$495.00
HPA-I
Mouse Apoptosis Primer Library
A ready-to-use Mouse Apoptosis PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against apoptosis genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 l - 10 M)

Perform up to 100 PCR arrays (based on 20 l assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:



Apaf1 apoptotic peptidase activating factor 1

Atm ataxia telangiectasia mutated homolog

Bad Bcl-associated death promoter

Bag1 Bcl2-associated athanogene 1

Bag3 Bcl2-associated athanogene 3

Bag4 BCL2-associated athanogene 4

Bak1 BCL2-antagonist/killer 1

Bax Bcl2-associated X protein

Bbc3 Bcl-2 binding component 3

Bcl10 B-cell leukemia/lymphoma 10

Bcl2 B-cell leukemia/lymphoma 2

Bcl2a1 B-cell leukemia/lymphoma 2 related protein A1d

Bcl2l1 Bcl2-like 1

Bcl2l11 BCL2-like 11

Bcl2l2 Bcl2-like 2

Bfar bifunctional apoptosis regulator

Bik Bcl2-interacting killer

Naip1 NLR family, apoptosis inhibitory protein 1

Birc2 baculoviral IAP repeat-containing 2

Birc3 baculoviral IAP repeat-containing 3

Xiap X-linked inhibitor of apoptosis

Birc5 baculoviral IAP repeat-containing 5

Birc6 baculoviral IAP repeat-containing 6

Bnip3 BCL2/adenovirus E1B interacting protein 1

Bre brain and reproductive organ-expressed protein

Bok Bcl-2-related ovarian killer protein

Casp1 caspase 1

Casp4 caspase 4, apoptosis-related cysteine peptidase

Casp2 caspase 2

Casp3 caspase 3

Casp6 caspase 6

Casp7 caspase 7

Casp8 caspase 8

Casp8ap2 caspase 8 associated protein 2

Casp9 caspase 9

Cflar CASP8 and FADD-like apoptosis regulator

Chek1 checkpoint kinase 1 homolog

Chek2 CHK2 checkpoint homolog

Cidea cell death-inducing DNA fragmentation factor, alpha subunit-like effector A

Cideb cell death-inducing DNA fragmentation factor, alpha subunit-like effector B

Cradd CASP2 and RIPK1 domain containing adaptor

Dapk1 death associated protein kinase 1

Dapk2 death-associated kinase 2

Dffa DNA fragmentation factor, alpha subunit

Dffb DNA fragmentation factor, beta subunit

Fadd Fas (TNFRSF6)-associated via death domain

Gadd45a growth arrest and DNA-damage-inducible 45 alpha

Hrk harakiri, BCL2 interacting protein

Ltbr lymphotoxin B receptor

Mcl1 myeloid cell leukemia sequence 1

Myd88 myeloid differentiation primary response gene 88

Ripk1 receptor (TNFRSF)-interacting serine-threonine kinase 1

Ripk2 receptor (TNFRSF)-interacting serine-threonine kinase 2

Rpa3 replication protein A3

Tank TRAF family member-associated Nf-kappa B activator

Tnf tumor necrosis factor

Tnfrsf10b tumor necrosis factor receptor superfamily, member 10b

Tnfrsf11a receptor activator of nuclear factor kappa B

Tnfrsf11b tumor necrosis factor receptor superfamily, member 11b

Tnfrsf14 tumor necrosis factor receptor superfamily, member 14

Tnfrsf17 tumor necrosis factor receptor superfamily, member 17

Tnfrsf1a tumor necrosis factor receptor superfamily, member 1a

Tnfrsf21 tumor necrosis factor receptor superfamily, member 21

Tnfrsf4 tumor necrosis factor receptor superfamily, member 4

Cd40 CD40 antigen

Tnfrsf9 tumor necrosis factor receptor superfamily, member 9

Tnfsf10 tumor necrosis factor receptor superfamily, member 10

Tnfsf11 tumor necrosis factor receptor superfamily, member 11

Tnfsf12 tumor necrosis factor (ligand) superfamily, member 13

Tnfsf13b tumor necrosis factor (ligand) superfamily, member 13b

Tnfsf14 tumor necrosis factor (ligand) superfamily, member 14

Tnfsf15 tumor necrosis factor (ligand) superfamily, member 15

Tnfsf18 tumor necrosis factor (ligand) superfamily, member 18

Tnfsf4 tumor necrosis factor (ligand) superfamily, member 4

Cd40lg CD40 ligand

Fasl Fas ligand (TNF superfamily, member 6)

Tnfsf8 tumor necrosis factor (ligand) superfamily, member 8

Tnfsf9 tumor necrosis factor (ligand) superfamily, member 9

Trp53 transformation related protein 53

Trp73 transformation related protein 73

Traf1 Tnf receptor-associated factor 1

Traf2 Tnf receptor-associated factor 2

Traf3 Tnf receptor-associated factor 3

Traf4 Tnf receptor associated factor

Traf5 Tnf receptor-associated factor 5

Traf6 Tnf receptor-associated factor 6

Trip TRAF-interacting protein

Diablo diablo homolog

Actb Actin, beta

B2m Beta-2-microglobulin

Gapd Glyceraldehyde-3-phosphate dehydrogenase

Gusb Glucuronidase, beta

Hprt1 Hypoxanthine phosphoribosyltransferase 1

Pgk Phosphoglycerate kinase 1

Ppia Peptidylprolyl isomerase A

Rpl13a Ribosomal protein L13a
$495.00
MPA-I