SYBR Green qPCR primers for detecting rat Cdc25b (cell division cycle 25B) gene expression by real-time PCR.
Rat Cdc25b qPCR Primer Set – cell division cycle 25B
About this gene: Cdc25b is predicted to enable protein kinase binding activity and protein tyrosine phosphatase activity. It is predicted to be involved in G2/M transition of mitotic cell cycle; positive regulation of cell cycle process; and protein phosphorylation. It is predicted to act upstream of or within female meiosis I; oocyte maturation; and positive regulation of protein kinase activity.
Gene details: NCBI Gene ID: 171103; UniProt: P48966; Chromosome location: 3q36
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.