SYBR Green qPCR primers for detecting rat Cast (calpastatin) gene expression by real-time PCR.
Rat Cast qPCR Primer Set – calpastatin
About this gene: Cast enables calcium-dependent cysteine-type endopeptidase inhibitor activity and protease binding activity. It is involved in multiple processes, including animal organ regeneration; myoblast fusion; and skeletal muscle atrophy.
Gene details: NCBI Gene ID: 25403; UniProt: P27321; Chromosome location: 2q11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.