SYBR Green qPCR primers for detecting rat Capn1 (calpain 1, also called muCANP) gene expression by real-time PCR.
Rat Capn1 qPCR Primer Set – calpain 1
Also known as: CANP 1, muCANP
About this gene: Capn1 enables multiple functions, including calcium ion binding activity; calcium-dependent cysteine-type endopeptidase activity; and cytoskeletal protein binding activity. It is involved in multiple processes, including negative regulation of non-canonical NF-kappaB signal transduction; positive regulation of cardiac muscle cell apoptotic process; and positive regulation of leukocyte tethering or rolling. Is active in glutamatergic synapse; postsynaptic density; and presynapse.
Gene details: NCBI Gene ID: 29153; UniProt: P97571; Chromosome location: 1q43
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.