SYBR Green qPCR primers for detecting rat Bub1b (BUB1 mitotic checkpoint serine/threonine kinase B) gene expression by real-time PCR.
Rat Bub1b qPCR Primer Set – BUB1 mitotic checkpoint serine/threonine kinase B
About this gene: Bub1b is predicted to enable protein kinase activity. It is predicted to be involved in meiotic sister chromatid cohesion, centromeric and mitotic spindle assembly checkpoint signaling. It is predicted to act upstream of or within metaphase/anaphase transition of mitotic cell cycle and protein localization to chromosome, centromeric region.
Gene details: NCBI Gene ID: 171576; Chromosome location: 3q35
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.