SYBR Green qPCR primers for detecting rat Brf2 (BRF2 general transcription factor 3B subunit) gene expression by real-time PCR.
Rat Brf2 qPCR Primer Set – BRF2 general transcription factor 3B subunit
About this gene: Brf2 is predicted to enable RNA polymerase II general transcription initiation factor activity; RNA polymerase III type 3 promoter sequence-specific DNA binding activity; and TBP-class protein binding activity. Predicted to be active in nucleus.
Gene details: NCBI Gene ID: 306542; UniProt: Q4V8D6; Chromosome location: 16q12.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.