SYBR Green qPCR primers for detecting rat Brca1 (BRCA1, DNA repair associated, also called RNF53) gene expression by real-time PCR.
Rat Brca1 qPCR Primer Set – BRCA1, DNA repair associated
Also known as: BRCA-1
About this gene: Brca1 enables chromatin binding activity. It is involved in multiple processes, including positive regulation of protein import into nucleus; response to estradiol; and response to genistein.
Gene details: NCBI Gene ID: 497672; UniProt: O54952; Chromosome location: 10q31
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.