SYBR Green qPCR primers for detecting rat Blm (BLM RecQ like helicase) gene expression by real-time PCR.
Rat Blm qPCR Primer Set – BLM RecQ like helicase
About this gene: Blm is predicted to enable multiple functions, including ATP binding activity; DNA binding activity; and DNA helicase activity. It is predicted to be involved in multiple processes, including DNA geometric change; DNA metabolic process; and cellular response to camptothecin. It is predicted to act upstream of or within multiple processes, including negative regulation of thymocyte apoptotic process; regulation of DNA recombination; and replication-born double-strand break repair via sister chromatid exchange.
Gene details: NCBI Gene ID: 308755; Chromosome location: 1q31
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.