SYBR Green qPCR primers for detecting rat Bckdhb (branched chain keto acid dehydrogenase E1 subunit beta, also called BCKDE1B) gene expression by real-time PCR.
Rat Bckdhb qPCR Primer Set – branched chain keto acid dehydrogenase E1 subunit beta
Also known as: BCKDE1B
About this gene: Predicted to contribute to branched-chain 2-oxo acid dehydrogenase activity. It is involved in response to cAMP; response to glucocorticoid; and response to nutrient. Predicted to be active in mitochondrial matrix.
Gene details: NCBI Gene ID: 29711; UniProt: P35738; Chromosome location: 8q31
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.