SYBR Green qPCR primers for detecting rat Auh (AU RNA binding methylglutaconyl-CoA hydratase) gene expression by real-time PCR.
Rat Auh qPCR Primer Set – AU RNA binding methylglutaconyl-CoA hydratase
About this gene: Auh is predicted to enable enoyl-CoA hydratase activity; mRNA 3'-UTR binding activity; and methylglutaconyl-CoA hydratase activity. It is predicted to be involved in L-leucine catabolic process and fatty acid beta-oxidation. Predicted to be active in mitochondrion.
Gene details: NCBI Gene ID: 361215; UniProt: F1LU71; Chromosome location: 17p14
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.