SYBR Green qPCR primers for detecting rat Aacs (acetoacetyl-CoA synthetase) gene expression by real-time PCR.
Rat Aacs qPCR Primer Set – acetoacetyl-CoA synthetase
About this gene: Aacs enables acetoacetate-CoA ligase activity and short-chain fatty acid-CoA ligase activity. It is involved in multiple processes, including cellular response to cholesterol; cellular response to testosterone stimulus; and positive regulation of insulin secretion.
Gene details: NCBI Gene ID: 65984; UniProt: Q9JMI1; Chromosome location: 12q14
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.