SYBR Green qPCR primers for detecting rat Aqp4 (aquaporin 4, also called MIWC) gene expression by real-time PCR.
Rat Aqp4 qPCR Primer Set – aquaporin 4
Also known as: AQP-4, Miwc, WCH4
About this gene: Aqp4 belongs to the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The Aqp4 protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. A recent study provided evidence for translational readthrough in Aqp4 and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
Gene details: NCBI Gene ID: 25293; UniProt: P47863; Chromosome location: 18p13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.