SYBR Green qPCR primers for detecting rat Aptx (aprataxin, also called FHA-HIT) gene expression by real-time PCR.
Rat Aptx qPCR Primer Set – aprataxin
About this gene: Aptx is predicted to enable multiple functions, including DNA 5'-adenosine monophosphate hydrolase activity; DNA binding activity; and phosphatase activity. It is predicted to be involved in regulation of protein stability and single strand break repair. Predicted to be active in nucleus.
Gene details: NCBI Gene ID: 259271; UniProt: Q8K4H4; Chromosome location: 5q22
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.