SYBR Green qPCR primers for detecting rat Apob (apolipoprotein B) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Rat Apob qPCR Primer Set – apolipoprotein B
Also known as: Aa1064, Ac1-060, Apo B-100, ApoB-100, ApoB-48
About this gene: Apob product is the main apolipoprotein of chylomicrons and low density lipoproteins. The Apob gene product occurs in plasma as two main isoforms, apoB-L and apoB-H. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence.
Gene details: NCBI Gene ID: 54225; UniProt: Q7TMA5; Chromosome location: 6q14
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Rat PCR Primer Sets.