SYBR Green qPCR primers for detecting rat Ap3b1 (adaptor related protein complex 3 subunit beta 1) gene expression by real-time PCR.
Rat Ap3b1 qPCR Primer Set – adaptor related protein complex 3 subunit beta 1
About this gene: Ap3b1 is predicted to enable AP-3 adaptor complex binding activity; GTP-dependent protein binding activity; and protein phosphatase binding activity. It is predicted to be involved in multiple processes, including T-helper 1 cell activation; anterograde synaptic vesicle transport; and response to Gram-positive bacterium. It is predicted to act upstream of or within with a negative effect on mRNA transcription by RNA polymerase II and regulation of catalytic activity.
Gene details: NCBI Gene ID: 309969; Chromosome location: 2q12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.