SYBR Green qPCR primers for detecting rat Alox12b (arachidonate 12-lipoxygenase, 12R type) gene expression by real-time PCR.
Rat Alox12b qPCR Primer Set – arachidonate 12-lipoxygenase, 12R type
About this gene: Alox12b is predicted to enable arachidonate 12-lipoxygenase activity; arachidonate 8-lipoxygenase activity; and linoleate 9S-lipoxygenase activity. It is involved in arachidonate metabolic process and hepoxilin biosynthetic process. Predicted to be active in perinuclear region of cytoplasm.
Gene details: NCBI Gene ID: 287425; UniProt: Q2KMM4; Chromosome location: 10q24
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.