SYBR Green qPCR primers for detecting rat Alox12 (arachidonate 12-lipoxygenase, 12S type) gene expression by real-time PCR.
Rat Alox12 qPCR Primer Set – arachidonate 12-lipoxygenase, 12S type
Also known as: 12-LO, 12S-LOX
About this gene: Alox12 is predicted to enable arachidonate 12-lipoxygenase activity; arachidonate 15-lipoxygenase activity; and linoleate 13S-lipoxygenase activity. It is involved in multiple processes, including fatty acid metabolic process; positive regulation of endothelial tube morphogenesis; and regulation of apoptotic process.
Gene details: NCBI Gene ID: 287454; UniProt: F1LQ70; Chromosome location: 10q24
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.