SYBR Green qPCR primers for detecting rat Alkbh7 (Alkbh7 RNA demethylase) gene expression by real-time PCR.
Rat Alkbh7 qPCR Primer Set – Alkbh7 RNA demethylase
About this gene: Alkbh7 is predicted to enable tRNA demethylase activity. It is predicted to be involved in multiple processes, including oxidative RNA demethylation; regulation of lipid storage; and regulation of mitochondrial membrane permeability involved in programmed necrotic cell death. Predicted to be active in mitochondrial matrix.
Gene details: NCBI Gene ID: 679944; Chromosome location: 9q11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.