SYBR Green qPCR primers for detecting rat Akap8 (A-kinase anchoring protein 8) gene expression by real-time PCR.
Rat Akap8 qPCR Primer Set – A-kinase anchoring protein 8
Also known as: Akap95
About this gene: Akap8 enables double-stranded DNA binding activity; protein kinase A regulatory subunit binding activity; and zinc ion binding activity. It is predicted to be involved in multiple processes, including cellular response to lipopolysaccharide; cellular response to prostaglandin E stimulus; and negative regulation of tumor necrosis factor production.
Gene details: NCBI Gene ID: 116633; UniProt: Q63014; Chromosome location: 7q11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.