SYBR Green qPCR primers for detecting rat Fads3 (fatty acid desaturase 3) gene expression by real-time PCR.
Rat Fads3 qPCR Primer Set – fatty acid desaturase 3
About this gene: Fads3 is predicted to enable oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water. It is predicted to be involved in lipid metabolic process. It is predicted to act upstream of or within gene expression and unsaturated fatty acid biosynthetic process.
Gene details: NCBI Gene ID: 286922; UniProt: Q8K1P9; Chromosome location: 1q43
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.