SYBR Green qPCR primers for detecting rat Fads1 (fatty acid desaturase 1, also called D5D) gene expression by real-time PCR.
Rat Fads1 qPCR Primer Set – fatty acid desaturase 1
About this gene: Fads1 enables acyl-CoA-desaturase activity. It is involved in multiple processes, including response to fenofibrate; response to vitamin A; and unsaturated fatty acid metabolic process. Predicted to be active in membrane.
Gene details: NCBI Gene ID: 84575; UniProt: Q920R3; Chromosome location: 1q43
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.