SYBR Green qPCR primers for detecting rat Espn (espin) gene expression by real-time PCR.
Rat Espn qPCR Primer Set – espin
Also known as: Je
About this gene: Espn enables SH3 domain binding activity and actin filament binding activity. It is predicted to be involved in microvillar actin bundle assembly and negative regulation of cytoskeleton organization. It is predicted to act upstream of or within locomotory behavior; parallel actin filament bundle assembly; and positive regulation of filopodium assembly.
Gene details: NCBI Gene ID: 56227; Chromosome location: 5q36
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.