SYBR Green qPCR primers for detecting rat Ercc3 (ERCC excision repair 3, TFIIH core complex helicase subunit) gene expression by real-time PCR.
Rat Ercc3 qPCR Primer Set – ERCC excision repair 3, TFIIH core complex helicase subunit
About this gene: Ercc3 is predicted to enable 3'-5' DNA helicase activity; ATP hydrolysis activity; and promoter-specific chromatin binding activity. It is involved in embryonic organ development and response to hypoxia.
Gene details: NCBI Gene ID: 291703; UniProt: Q4G005; Chromosome location: 18p12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.