SYBR Green qPCR primers for detecting rat Epm2a (EPM2A glucan phosphatase, laforin, also called LAFPTPase) gene expression by real-time PCR.
Rat Epm2a qPCR Primer Set – EPM2A glucan phosphatase, laforin
About this gene: Epm2a is predicted to enable phosphatase activity; polysaccharide binding activity; and protein homodimerization activity. It is predicted to be involved in multiple processes, including carbohydrate phosphorylation; negative regulation of phosphatase activity; and peptidyl-tyrosine dephosphorylation. It is predicted to act upstream of or within multiple processes, including glycogen biosynthetic process; habituation; and regulation of protein metabolic process.
Gene details: NCBI Gene ID: 114005; UniProt: Q91XQ2; Chromosome location: 1p13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.