SYBR Green qPCR primers for detecting rat Epas1 (endothelial PAS domain protein 1, also called HIF2-alpha) gene expression by real-time PCR.
Rat Epas1 qPCR Primer Set – endothelial PAS domain protein 1
Also known as: HIF-2 alpha, HIF2 alpha, HLF, Hif2a
About this gene: Epas1 enables DNA-binding transcription factor activity and cobalt ion binding activity. Contributes to sequence-specific DNA binding activity. Predicted to be active in nucleus.
Gene details: NCBI Gene ID: 29452; UniProt: Q9JHS1; Chromosome location: 6q12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.