SYBR Green qPCR primers for detecting rat En1 (engrailed homeobox 1) gene expression by real-time PCR.
Rat En1 qPCR Primer Set – engrailed homeobox 1
About this gene: En1 is predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. It is involved in response to cocaine. Predicted to be active in nucleus.
Gene details: NCBI Gene ID: 685360; Chromosome location: 13q11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.