SYBR Green qPCR primers for detecting rat Eif5a (eukaryotic translation initiation factor 5A, also called eIF-5A) gene expression by real-time PCR.
Rat Eif5a qPCR Primer Set – eukaryotic translation initiation factor 5A
About this gene: Eif5a is predicted to enable U6 snRNA binding activity and translation elongation factor activity. It is involved in multiple processes, including cellular response to thyroid hormone stimulus; positive regulation of reactive oxygen species metabolic process; and regulation of apoptotic process. Is active in synapse.
Gene details: NCBI Gene ID: 287444; UniProt: Q3T1J1; Chromosome location: 10q24
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.