SYBR Green qPCR primers for detecting rat Eif4g2 (eukaryotic translation initiation factor 4, gamma 2) gene expression by real-time PCR.
Rat Eif4g2 qPCR Primer Set – eukaryotic translation initiation factor 4, gamma 2
About this gene: Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F, which is a cap binding protein complex that consists of three subunits: eIF4A. Unlike eIF4G, which supports cap-dependent and independent translation, Eif4g2 product acts as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies in human indicate that translation of this mRNA initiates exclusively at a non-AUG codon.
Gene details: NCBI Gene ID: 361628; Chromosome location: 1q33
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.