SYBR Green qPCR primers for detecting rat Ago3 (argonaute RISC catalytic component 3) gene expression by real-time PCR.
Rat Ago3 qPCR Primer Set – argonaute RISC catalytic component 3
Also known as: Eif2c3
About this gene: Ago3 is predicted to enable RNA binding activity and endoribonuclease activity, cleaving miRNA-paired mRNA. Predicted to be involved in multiple processes, including negative regulation of gene expression; positive regulation of non-canonical NF-kappaB signal transduction. Predicted to be active in cytoplasmic ribonucleoprotein granule and nucleus.
Gene details: NCBI Gene ID: 313593; Chromosome location: 5q36
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.