SYBR Green qPCR primers for detecting rat Ehhadh (enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase, also called PBE) gene expression by real-time PCR.
Rat Ehhadh qPCR Primer Set – enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase
Also known as: Lbp, MEF, MFP1, Mfe, Mfe1, Pbe, Pbfe, pe-CoA, perMFE-1
About this gene: Ehhadh enables multiple functions, including-3-hydroxyacyl-CoA dehydrogenase activity; delta-delta-enoyl-CoA isomerase activity; and enoyl-CoA hydratase activity. It is involved in fatty acid beta-oxidation.
Gene details: NCBI Gene ID: 171142; UniProt: P07896; Chromosome location: 11q23
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.