SYBR Green qPCR primers for detecting rat Egr1 (early growth response 1, also called NGFI-A) gene expression by real-time PCR.
Rat Egr1 qPCR Primer Set – early growth response 1
Also known as: Krox-24, NGFI-A, Ngf1, Ngfi, zif-268
About this gene: Egr1 enables DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II transcription regulatory region sequence-specific DNA binding activity; and promoter-specific chromatin binding activity. It is involved in multiple processes, including positive regulation of cell population proliferation; regulation of synaptic plasticity; and response to isoquinoline alkaloid.
Gene details: NCBI Gene ID: 24330; UniProt: P08154; Chromosome location: 18p12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.